ABCMdb

PMID: 17977821

Demirel O, Waibler Z, Kalinke U, Grunebach F, Appel S, Brossart P, Hasilik A, Tampe R, Abele R
Identification of a lysosomal peptide transport system induced during dendritic cell development.
J Biol Chem. 2007 Dec 28;282(52):37836-43. Epub 2007 Oct 31., [PubMed]

Sentences

No. Mutations Sentence Comment

47 ABCB9 p.His699Ala ABCB9 p.Lys545Ala TAPL-K545A/ H699A was generated by Ligase Chain Reaction using the primers 5Ј-CGGGCAGTGGGGCGAGCTCCTGTGTC-3Ј and 5Ј-CTC- ATCATCGCGGCCCGGCTGAGCAC-3Ј (mutated bases are underlined). Login to comment 75 ABCB9 p.His699Ala ABCB9 p.Lys545Ala Retroviral Transduction-Recombinant retroviruses were obtained by co-transfection of pCL-Ampho (generous gift of Peter Cresswell) (28), and the retroviral constructs pLPCX- TAPL or pLPCX-TAPL-K545A/H699A into 293T cells with Lipofectamine 2000 (Invitrogen) following the manufacturer`s instructions. Login to comment 198 ABCB9 p.His699Ala ABCB9 p.Lys545Ala After retroviral transduction, TAPL and the Walker A/H-loop mutant K545A/H699A were detected by immunoblotting as single bands (Fig. 4A). Login to comment 213 ABCB9 p.His699Ala ABCB9 p.Lys545Ala Perinuclear supernatants (PNS) derivedfromTAPL-wt-orTAPL-K545A/H699A-transducedRajicellswerefrac- tioned by a Percoll gradient and analyzed by SDS-PAGE and immunoblotting. Login to comment