ABCB6 p.Gly628Ala
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PMID: 10837493
[PubMed]
Mitsuhashi N et al: "MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis."
No.
Sentence
Comment
67
Analysis of MTABC3 Function-The Walker A-encoding region of MTABC3 cDNA was mutated by a PCR-based method using oligonucleotides bearing mismatched bases at the residues to be mutated (5Ј-CCA TCT GGG GCA GCG AGG AGC ACA ATT TTG-3Ј and 5Ј-CAA AAT TGT GCT CCT CGC TGC CCC AGA TGG-3Ј for G628A and K629R, respectively) in combination with oligonucleotides (5Ј-GTT ACC AGT TAC GTC TTC CT-3Ј and 5Ј-TCT TTG AGA GGG AAG TGG CC-3Ј) (24).
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ABCB6 p.Gly628Ala 10837493:67:309
status: NEW128 We then mutated the Walker A motif, GPSGAGKST of MTABC3 to GPSGAARST, and the resultant double mutant, MTABC3 (G628A, K629R), was introduced into atm1-1 mutant cells.
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ABCB6 p.Gly628Ala 10837493:128:111
status: NEW139 In addition, only 30% of atm1-1 mutant cells transformed with the double mutant MTABC3 (G628A, K629R) maintained respiratory function.
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ABCB6 p.Gly628Ala 10837493:139:88
status: NEW149 Interestingly, although the iron accumulation is only partially reversed (Fig. 3A), the damage of mitochondrial DNA (Fig. 3B) is almost fully reversed by the double mutant MTABC3 (G628A, K629R) in atm1-1 mutant cells.
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ABCB6 p.Gly628Ala 10837493:149:180
status: NEW171 A, iron content of mitochondria isolated from yeast YM13-1c strain transformed with pYES2, ATM1, MTABC3, or MTABC3 double mutant (G628A, K629R) (values are mean Ϯ S.E.).
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ABCB6 p.Gly628Ala 10837493:171:130
status: NEW174 C, the rates (%) of cells maintaining mitochondrial respiratory function obtained by transforming pYES2 vector (Ⅺ), ATM1 (f), MTABC3 (⅜), or MTABC3 double mutant G628A, K629R (q) into atm1-1 mutant cells at the indicated times are shown (mean Ϯ S.E.).
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ABCB6 p.Gly628Ala 10837493:174:176
status: NEW66 Analysis of MTABC3 Function-The Walker A-encoding region of MTABC3 cDNA was mutated by a PCR-based method using oligonucleotides bearing mismatched bases at the residues to be mutated (5b18;-CCA TCT GGG GCA GCG AGG AGC ACA ATT TTG-3b18; and 5b18;-CAA AAT TGT GCT CCT CGC TGC CCC AGA TGG-3b18; for G628A and K629R, respectively) in combination with oligonucleotides (5b18;-GTT ACC AGT TAC GTC TTC CT-3b18; and 5b18;-TCT TTG AGA GGG AAG TGG CC-3b18;) (24).
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ABCB6 p.Gly628Ala 10837493:66:309
status: NEW127 We then mutated the Walker A motif, GPSGAGKST of MTABC3 to GPSGAARST, and the resultant double mutant, MTABC3 (G628A, K629R), was introduced into atm1-1 mutant cells.
X
ABCB6 p.Gly628Ala 10837493:127:111
status: NEW138 In addition, only 30% of atm1-1 mutant cells transformed with the double mutant MTABC3 (G628A, K629R) maintained respiratory function.
X
ABCB6 p.Gly628Ala 10837493:138:88
status: NEW148 Interestingly, although the iron accumulation is only partially reversed (Fig. 3A), the damage of mitochondrial DNA (Fig. 3B) is almost fully reversed by the double mutant MTABC3 (G628A, K629R) in atm1-1 mutant cells.
X
ABCB6 p.Gly628Ala 10837493:148:180
status: NEW170 A, iron content of mitochondria isolated from yeast YM13-1c strain transformed with pYES2, ATM1, MTABC3, or MTABC3 double mutant (G628A, K629R) (values are mean afe; S.E.).
X
ABCB6 p.Gly628Ala 10837493:170:130
status: NEW173 C, the rates (%) of cells maintaining mitochondrial respiratory function obtained by transforming pYES2 vector (ǧa;), ATM1 (f), MTABC3 (Ǧc;), or MTABC3 double mutant G628A, K629R (cf;) into atm1-1 mutant cells at the indicated times are shown (mean afe; S.E.).
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ABCB6 p.Gly628Ala 10837493:173:174
status: NEW