ABCMdb

PMID: 10837493

Mitsuhashi N, Miki T, Senbongi H, Yokoi N, Yano H, Miyazaki M, Nakajima N, Iwanaga T, Yokoyama Y, Shibata T, Seino S
MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis.
J Biol Chem. 2000 Jun 9;275(23):17536-40., [PubMed]

Sentences

No. Mutations Sentence Comment

66 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg Analysis of MTABC3 Function-The Walker A-encoding region of MTABC3 cDNA was mutated by a PCR-based method using oligonucleotides bearing mismatched bases at the residues to be mutated (5b18;-CCA TCT GGG GCA GCG AGG AGC ACA ATT TTG-3b18; and 5b18;-CAA AAT TGT GCT CCT CGC TGC CCC AGA TGG-3b18; for G628A and K629R, respectively) in combination with oligonucleotides (5b18;-GTT ACC AGT TAC GTC TTC CT-3b18; and 5b18;-TCT TTG AGA GGG AAG TGG CC-3b18;) (24). Login to comment 67 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg Analysis of MTABC3 Function-The Walker A-encoding region of MTABC3 cDNA was mutated by a PCR-based method using oligonucleotides bearing mismatched bases at the residues to be mutated (5Ј-CCA TCT GGG GCA GCG AGG AGC ACA ATT TTG-3Ј and 5Ј-CAA AAT TGT GCT CCT CGC TGC CCC AGA TGG-3Ј for G628A and K629R, respectively) in combination with oligonucleotides (5Ј-GTT ACC AGT TAC GTC TTC CT-3Ј and 5Ј-TCT TTG AGA GGG AAG TGG CC-3Ј) (24). Login to comment 127 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg We then mutated the Walker A motif, GPSGAGKST of MTABC3 to GPSGAARST, and the resultant double mutant, MTABC3 (G628A, K629R), was introduced into atm1-1 mutant cells. Login to comment 128 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg We then mutated the Walker A motif, GPSGAGKST of MTABC3 to GPSGAARST, and the resultant double mutant, MTABC3 (G628A, K629R), was introduced into atm1-1 mutant cells. Login to comment 138 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg In addition, only 30% of atm1-1 mutant cells transformed with the double mutant MTABC3 (G628A, K629R) maintained respiratory function. Login to comment 139 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg In addition, only 30% of atm1-1 mutant cells transformed with the double mutant MTABC3 (G628A, K629R) maintained respiratory function. Login to comment 148 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg Interestingly, although the iron accumulation is only partially reversed (Fig. 3A), the damage of mitochondrial DNA (Fig. 3B) is almost fully reversed by the double mutant MTABC3 (G628A, K629R) in atm1-1 mutant cells. Login to comment 149 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg Interestingly, although the iron accumulation is only partially reversed (Fig. 3A), the damage of mitochondrial DNA (Fig. 3B) is almost fully reversed by the double mutant MTABC3 (G628A, K629R) in atm1-1 mutant cells. Login to comment 170 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg A, iron content of mitochondria isolated from yeast YM13-1c strain transformed with pYES2, ATM1, MTABC3, or MTABC3 double mutant (G628A, K629R) (values are mean afe; S.E.). Login to comment 171 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg A, iron content of mitochondria isolated from yeast YM13-1c strain transformed with pYES2, ATM1, MTABC3, or MTABC3 double mutant (G628A, K629R) (values are mean Ϯ S.E.). Login to comment 173 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg C, the rates (%) of cells maintaining mitochondrial respiratory function obtained by transforming pYES2 vector (ǧa;), ATM1 (f), MTABC3 (Ǧc;), or MTABC3 double mutant G628A, K629R (cf;) into atm1-1 mutant cells at the indicated times are shown (mean afe; S.E.). Login to comment 174 ABCB6 p.Gly628Ala ABCB6 p.Lys629Arg C, the rates (%) of cells maintaining mitochondrial respiratory function obtained by transforming pYES2 vector (Ⅺ), ATM1 (f), MTABC3 (⅜), or MTABC3 double mutant G628A, K629R (q) into atm1-1 mutant cells at the indicated times are shown (mean Ϯ S.E.). Login to comment