ABCB2 p.Ser105Cys
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PMID: 22447242
[PubMed]
Hohl M et al: "Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation."
No.
Sentence
Comment
430
A cysteine-less TM287/288 clone (see Supplementary Methods) served as template for the introduction of the -single mutants TM287(S105C) and TM288(T486C) and the corresponding double mutant.MutantTM287/288waspurifiedandincubatedwithcopperphenanthroline (1mM)andnucleotides(2.5mM)asindicatedinBufferAcontaining3mMMgCl2 at roomtemperaturefor20min.Thecross-linkedproteinwasseparatedbynonreducing SDS-PAGE and quantified by SYPRO ruby staining (Invitrogen).
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ABCB2 p.Ser105Cys 22447242:430:129
status: NEW