PMID: 22447242

Hohl M, Briand C, Grutter MG, Seeger MA
Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation.
Nat Struct Mol Biol. 2012 Mar 25;19(4):395-402. doi: 10.1038/nsmb.2267., [PubMed]
Sentences
No. Mutations Sentence Comment
430 ABCB2 p.Ser105Cys
X
ABCB2 p.Ser105Cys 22447242:430:129
status: NEW
view ABCB2 p.Ser105Cys details
 A cysteine-less TM287/288 clone (see Supplementary Methods) served as template for the introduction of the -single mutants TM287(S105C) and TM288(T486C) and the corresponding double mutant.MutantTM287/288waspurifiedandincubatedwithcopperphenanthroline (1mM)andnucleotides(2.5mM)asindicatedinBufferAcontaining3mMMgCl2 at roomtemperaturefor20min.Thecross-linkedproteinwasseparatedbynonreducing SDS-PAGE and quantified by SYPRO ruby staining (Invitrogen). Login to comment