ABCA1 p.Tyr924Phe
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PMID: 19783654
[PubMed]
Tang C et al: "The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor."
No.
Sentence
Comment
42
Cells were selected against Neozi, and clonal lines expressing similar amounts of wild-type (WT) ABCA1, mutants Y924F, Y1990F, or Y924/1990F were used for the experiments.
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ABCA1 p.Tyr924Phe 19783654:42:112
status: NEW112 Probing immunoprecipitates of STAT3 with an ABCA1 antibody revealed that the apoA-I-stimulated interaction of ABCA1 with STAT3 was completely abolished by the Y924F/ Y1990F mutations (Fig. 3C).
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ABCA1 p.Tyr924Phe 19783654:112:24
status: NEWX
ABCA1 p.Tyr924Phe 19783654:112:159
status: NEW114 A lack of effect of the Y924F and Y1990F mutations on cholesterol efflux despite a loss of STAT3 phosphorylation (Fig. 3, A and B) indicates that the ABCA1-dependent activation of STAT3 plays no role in the cholesterol export function of ABCA1.
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ABCA1 p.Tyr924Phe 19783654:114:24
status: NEW128 A, mifepristone-treated BHK cells transfected with wild-type (WT) ABCA1 or ABCA1 containing Y924F, Y1990F, or Y924F/Y1990F substitutions were labeled with [3 H]cholesterol and incubated for 2 h without or with 10 g/ml apoA-I, and cholesterol efflux was calculated as the apoA-I-mediated release of [3 H]cholesterol into the medium normalized for values for WT cells.
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ABCA1 p.Tyr924Phe 19783654:128:92
status: NEWX
ABCA1 p.Tyr924Phe 19783654:128:110
status: NEW133 C, whole cell lysates from BHK cells transfected with WT and Y924F/Y1990F were immunoprecipitated with anti-STAT3 antibody and immunoblotted with anti-ABCA1 antibody.
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ABCA1 p.Tyr924Phe 19783654:133:61
status: NEW41 Cells were selected against Neozi, and clonal lines expressing similar amounts of wild-type (WT) ABCA1, mutants Y924F, Y1990F, or Y924/1990F were used for the experiments.
X
ABCA1 p.Tyr924Phe 19783654:41:112
status: NEW110 Probing immunoprecipitates of STAT3 with an ABCA1 antibody revealed that the apoA-I-stimulated interaction of ABCA1 with STAT3 was completely abolished by the Y924F/ Y1990F mutations (Fig. 3C).
X
ABCA1 p.Tyr924Phe 19783654:110:159
status: NEW126 A, mifepristone-treated BHK cells transfected with wild-type (WT) ABCA1 or ABCA1 containing Y924F, Y1990F, or Y924F/Y1990F substitutions were labeled with [3 H]cholesterol and incubated for 2 h without or with 10 òe;g/ml apoA-I, and cholesterol efflux was calculated as the apoA-I-mediated release of [3 H]cholesterol into the medium normalized for values for WT cells.
X
ABCA1 p.Tyr924Phe 19783654:126:92
status: NEWX
ABCA1 p.Tyr924Phe 19783654:126:110
status: NEW131 C, whole cell lysates from BHK cells transfected with WT and Y924F/Y1990F were immunoprecipitated with anti-STAT3 antibody and immunoblotted with anti-ABCA1 antibody.
X
ABCA1 p.Tyr924Phe 19783654:131:61
status: NEW