ABCMdb

PMID: 19783654

Tang C, Liu Y, Kessler PS, Vaughan AM, Oram JF
The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor.
J Biol Chem. 2009 Nov 20;284(47):32336-43. Epub 2009 Sep 25., [PubMed]

Sentences

No. Mutations Sentence Comment

41 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe Cells were selected against Neozi, and clonal lines expressing similar amounts of wild-type (WT) ABCA1, mutants Y924F, Y1990F, or Y924/1990F were used for the experiments. Login to comment 42 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe Cells were selected against Neozi, and clonal lines expressing similar amounts of wild-type (WT) ABCA1, mutants Y924F, Y1990F, or Y924/1990F were used for the experiments. Login to comment 110 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe Probing immunoprecipitates of STAT3 with an ABCA1 antibody revealed that the apoA-I-stimulated interaction of ABCA1 with STAT3 was completely abolished by the Y924F/ Y1990F mutations (Fig. 3C). Login to comment 112 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe ABCA1 p.Tyr924Phe Probing immunoprecipitates of STAT3 with an ABCA1 antibody revealed that the apoA-I-stimulated interaction of ABCA1 with STAT3 was completely abolished by the Y924F/ Y1990F mutations (Fig. 3C). Login to comment 114 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe A lack of effect of the Y924F and Y1990F mutations on cholesterol efflux despite a loss of STAT3 phosphorylation (Fig. 3, A and B) indicates that the ABCA1-dependent activation of STAT3 plays no role in the cholesterol export function of ABCA1. Login to comment 126 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe ABCA1 p.Tyr924Phe A, mifepristone-treated BHK cells transfected with wild-type (WT) ABCA1 or ABCA1 containing Y924F, Y1990F, or Y924F/Y1990F substitutions were labeled with [3 H]cholesterol and incubated for 2 h without or with 10 òe;g/ml apoA-I, and cholesterol efflux was calculated as the apoA-I-mediated release of [3 H]cholesterol into the medium normalized for values for WT cells. Login to comment 128 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe ABCA1 p.Tyr924Phe A, mifepristone-treated BHK cells transfected with wild-type (WT) ABCA1 or ABCA1 containing Y924F, Y1990F, or Y924F/Y1990F substitutions were labeled with [3 H]cholesterol and incubated for 2 h without or with 10 ␮g/ml apoA-I, and cholesterol efflux was calculated as the apoA-I-mediated release of [3 H]cholesterol into the medium normalized for values for WT cells. Login to comment 131 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe C, whole cell lysates from BHK cells transfected with WT and Y924F/Y1990F were immunoprecipitated with anti-STAT3 antibody and immunoblotted with anti-ABCA1 antibody. Login to comment 133 ABCA1 p.Tyr1990Phe ABCA1 p.Tyr924Phe C, whole cell lysates from BHK cells transfected with WT and Y924F/Y1990F were immunoprecipitated with anti-STAT3 antibody and immunoblotted with anti-ABCA1 antibody. Login to comment