ABCC8 p.Gln1372Ala
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 22187380
[PubMed]
Fatehi M et al: "The ATP-sensitive K(+) channel ABCC8 S1369A type 2 diabetes risk variant increases MgATPase activity."
No.
Sentence
Comment
52
The E23K/S1369A variants and the Q1372A mutation were introduced into the KCNJ11 and ABCC8 cDNAs, respectively, using site-directed mutagenesis (QuikChange; Stratagene, La Jolla, CA) and confirmed by sequence analysis.
X
ABCC8 p.Gln1372Ala 22187380:52:33
status: NEW91 Accordingly, we introduced the Q1372A mutation in the presence of S1369 (E23/S1369-Q1372A) to test whether the GTP stimulatory effect is similarly enhanced.
X
ABCC8 p.Gln1372Ala 22187380:91:31
status: NEWX
ABCC8 p.Gln1372Ala 22187380:91:83
status: NEW92 The Q1372A mutation significantly enhanced the stimulatory effect of GTP compared with KATP channels containing E23/S1369 and Q1372 (2.42 6 0.15vs. 1.83 6 0.14-fold, P , 0.0001) (Fig. 2D and E).
X
ABCC8 p.Gln1372Ala 22187380:92:4
status: NEW102 E: Grouped data showing a significant increase in the stimulatory effect of GTP in channels containing the A1369 variant and in channels containing the E23/S1369 variants with the Q1372A mutation (n = 10-20 patches per group).
X
ABCC8 p.Gln1372Ala 22187380:102:180
status: NEW155 This concept is supported further by our data showing that introduction of the Q1372A mutation into channels containing the S1369 variant enhanced the GTP stimulatory effect (Fig. 2D and E).
X
ABCC8 p.Gln1372Ala 22187380:155:79
status: NEW