ABCMdb

PMID: 22187380

Fatehi M, Raja M, Carter C, Soliman D, Holt A, Light PE
The ATP-sensitive K(+) channel ABCC8 S1369A type 2 diabetes risk variant increases MgATPase activity.
Diabetes. 2012 Jan;61(1):241-9., [PubMed]

Sentences

No. Mutations Sentence Comment

52 ABCC8 p.Gln1372Ala The E23K/S1369A variants and the Q1372A mutation were introduced into the KCNJ11 and ABCC8 cDNAs, respectively, using site-directed mutagenesis (QuikChange; Stratagene, La Jolla, CA) and confirmed by sequence analysis. Login to comment 91 ABCC8 p.Gln1372Ala ABCC8 p.Gln1372Ala Accordingly, we introduced the Q1372A mutation in the presence of S1369 (E23/S1369-Q1372A) to test whether the GTP stimulatory effect is similarly enhanced. Login to comment 92 ABCC8 p.Gln1372Ala The Q1372A mutation significantly enhanced the stimulatory effect of GTP compared with KATP channels containing E23/S1369 and Q1372 (2.42 6 0.15vs. 1.83 6 0.14-fold, P , 0.0001) (Fig. 2D and E). Login to comment 102 ABCC8 p.Gln1372Ala E: Grouped data showing a significant increase in the stimulatory effect of GTP in channels containing the A1369 variant and in channels containing the E23/S1369 variants with the Q1372A mutation (n = 10-20 patches per group). Login to comment 155 ABCC8 p.Gln1372Ala This concept is supported further by our data showing that introduction of the Q1372A mutation into channels containing the S1369 variant enhanced the GTP stimulatory effect (Fig. 2D and E). Login to comment