ABCC8 p.Cys418Ala
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PMID: 9658200
[PubMed]
Bouzat C et al: "Mutations at lipid-exposed residues of the acetylcholine receptor affect its gating kinetics."
No.
Sentence
Comment
103
A minor component of 1.03 Ϯ 0.16 msec (relative area, 0.20 Ϯ 0.03) was observed in 40% of the recordings from C418A-containing channels.
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ABCC8 p.Cys418Ala 9658200:103:122
status: NEW105 ␣ Subunit on burst n/b Current n msec pA Adult (-containing) AChR Wild-type 0.98 Ϯ 0.23 1.25 Ϯ 0.19 1.08 Ϯ 0.10 5.70 Ϯ 0.30 6 M4ϫ4 0.23 Ϯ 0.02a 0.29 Ϯ 0.10a 1.09 Ϯ 0.05 5.50 Ϯ 0.20 6 L411A 0.93 Ϯ 0.16 1.06 Ϯ 0.15 1.12 Ϯ 0.10 5.60 Ϯ 0.10 5 M415A 1.30 Ϯ 0.25 1.49 Ϯ 0.21 1.10 Ϯ 0.02 5.80 Ϯ 0.12 3 C418A 0.31 Ϯ 0.07a 0.36 Ϯ 0.07a 1.03 Ϯ 0.02 5.80 Ϯ 0.10 5 T422A 0.18 Ϯ 0.03a 0.20 Ϯ 0.04a 1.04 Ϯ 0.03 5.70 Ϯ 0.24 6 F414A 1.34 Ϯ 0.30 1.49 Ϯ 0.40 1.08 Ϯ 0.06 5.80 Ϯ 0.25 8 Embryonic (␥-containing) AChR Wild-type 5.51 Ϯ 0.51 9.20 Ϯ 1.41 1.70 Ϯ 0.14 3.85 Ϯ 0.13 6 C418A 2.82 Ϯ 0.56a 4.03 Ϯ 0.90a 1.28 Ϯ 0.10 3.67 Ϯ 0.14 6 T422A 1.55 Ϯ 0.03a 1.80 Ϯ 0.05a 1.20 Ϯ 0.10 4.00 Ϯ 0.20 6 a Statistically different from the wild-type value (p Ͻ 0.001).
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ABCC8 p.Cys418Ala 9658200:105:428
status: NEW126 When T422A or C418A mutant ␣ Fig. 2.
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ABCC8 p.Cys418Ala 9658200:126:14
status: NEW147 To determine whether the mutations in ␣M4 introduced changes in equilibrium agonist binding, we compared the inhibition of ␣-BTX binding by the agonist CCh in the two ␣M4 mutants, C418A, and T422A.
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ABCC8 p.Cys418Ala 9658200:147:201
status: NEW154 The largest decrease was observed with the C418A mutant (Fig. 6).
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ABCC8 p.Cys418Ala 9658200:154:43
status: NEW216 The curves are fits to the Hill equation with the following parameters: ␣2beta⑀␦: Kd ϭ 2 ϫ 10-5 M, nH ϭ 1.18; ␣2(C418A)beta⑀␦: Kd ϭ 2 ϫ 10-5 M, nH ϭ 1.10; ␣2(T422A)beta⑀␦: Kd ϭ 6 ϫ 10-5 M, nH ϭ 1.23.
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ABCC8 p.Cys418Ala 9658200:216:159
status: NEW