ABCMdb

PMID: 9658200

Bouzat C, Roccamo AM, Garbus I, Barrantes FJ
Mutations at lipid-exposed residues of the acetylcholine receptor affect its gating kinetics.
Mol Pharmacol. 1998 Jul;54(1):146-53., [PubMed]

Sentences

No. Mutations Sentence Comment

103 ABCC8 p.Cys418Ala A minor component of 1.03 Ϯ 0.16 msec (relative area, 0.20 Ϯ 0.03) was observed in 40% of the recordings from C418A-containing channels. Login to comment 105 ABCC8 p.Cys418Ala ␣ Subunit ␶on ␶burst n/b Current n msec pA Adult (␧-containing) AChR Wild-type 0.98 Ϯ 0.23 1.25 Ϯ 0.19 1.08 Ϯ 0.10 5.70 Ϯ 0.30 6 M4ϫ4 0.23 Ϯ 0.02a 0.29 Ϯ 0.10a 1.09 Ϯ 0.05 5.50 Ϯ 0.20 6 L411A 0.93 Ϯ 0.16 1.06 Ϯ 0.15 1.12 Ϯ 0.10 5.60 Ϯ 0.10 5 M415A 1.30 Ϯ 0.25 1.49 Ϯ 0.21 1.10 Ϯ 0.02 5.80 Ϯ 0.12 3 C418A 0.31 Ϯ 0.07a 0.36 Ϯ 0.07a 1.03 Ϯ 0.02 5.80 Ϯ 0.10 5 T422A 0.18 Ϯ 0.03a 0.20 Ϯ 0.04a 1.04 Ϯ 0.03 5.70 Ϯ 0.24 6 F414A 1.34 Ϯ 0.30 1.49 Ϯ 0.40 1.08 Ϯ 0.06 5.80 Ϯ 0.25 8 Embryonic (␥-containing) AChR Wild-type 5.51 Ϯ 0.51 9.20 Ϯ 1.41 1.70 Ϯ 0.14 3.85 Ϯ 0.13 6 C418A 2.82 Ϯ 0.56a 4.03 Ϯ 0.90a 1.28 Ϯ 0.10 3.67 Ϯ 0.14 6 T422A 1.55 Ϯ 0.03a 1.80 Ϯ 0.05a 1.20 Ϯ 0.10 4.00 Ϯ 0.20 6 a Statistically different from the wild-type value (p Ͻ 0.001). Login to comment 126 ABCC8 p.Cys418Ala When T422A or C418A mutant ␣ Fig. 2. Login to comment 147 ABCC8 p.Cys418Ala To determine whether the mutations in ␣M4 introduced changes in equilibrium agonist binding, we compared the inhibition of ␣-BTX binding by the agonist CCh in the two ␣M4 mutants, C418A, and T422A. Login to comment 154 ABCC8 p.Cys418Ala The largest decrease was observed with the C418A mutant (Fig. 6). Login to comment 216 ABCC8 p.Cys418Ala The curves are fits to the Hill equation with the following parameters: ␣2beta⑀␦: Kd ϭ 2 ϫ 10-5 M, nH ϭ 1.18; ␣2(C418A)beta⑀␦: Kd ϭ 2 ϫ 10-5 M, nH ϭ 1.10; ␣2(T422A)beta⑀␦: Kd ϭ 6 ϫ 10-5 M, nH ϭ 1.23. Login to comment