ABCC1 p.Gly5Glu
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PMID: 9334225
[PubMed]
Kast C et al: "Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence."
No.
Sentence
Comment
63
Cell Culture-Drug-sensitive human HeLa cells (ATCC) were grown in Dulbecco`s modified Eagle`s medium (high glucose) supplemented TABLE I Oligonucleotides used for epitope insertion by site-directed mutagenesis 1 Arg-4b (HA epitope)c Gly-5 Glu-127 (HA epitope) Arg-128 2 (363)a C TTT TTA ATT CAG CTG GAG AGG AGG AAG GGA GTT CAG (399) Glu-163 (HA epitope) Asp-164 3 (474) G ACA GCC TTA AAA GAG GAT GCC CAG GTG GAC (504) Gln-271 (HA epitope) Pro-272 4 (799) AAG ACTD AGG AAG CAG CCG GTG AAG GTT GTG TA (830) Ser-417 (HA epitope) Thr-419 5 (1233) C AAT TCA GCC AGA AAA TCC ACG GTC GGG GAG ATT G (1270) Asn-461 (HA epitope) Leu-462 6a (1369) CTC CTG TGG CTG AAT CTG GGC CCT TCC GTC (1398) 6b Asn-461 (HA epitope) ϫ 2 Leu-462 Leu-529 (HA epitope) Ala-530 7 (1572) C AAG GAC AAG GTG CTG GCT ATC AGG CAG GAG GA (1604) Asn-574 (HA epitope) Asn-575 8a (1707) G ACC ATT GAC GAG AAC AAT ATC CTG GAT GCC CA (1739) 8b Asn-574 (HA epitope) ϫ 2 Asn-575 9 Trp-653 (HA epitope) Ala-654 (HA epitope)c :Y P Y D V P D Y A S a Nucleotide position in sequence of MRP published by Cole et al. (10; 55).
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ABCC1 p.Gly5Glu 9334225:63:233
status: NEW