ABCB1 p.Asp744Arg
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PMID: 9725920
[PubMed]
Moss K et al: "Coupled translocation events generate topological heterogeneity at the endoplasmic reticulum membrane."
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Sentence
Comment
62
We then used the PCR overlap extension method to introduced mutations D743R/D744R.
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ABCB1 p.Asp744Arg 9725920:62:76
status: NEW73 D743R/ D744R and ⌬NGGLQP mutations were subsequently engineered into pSPMDR1-P by ligating HindIII-BstEII fragments from plasmids TM7a/b-8(DD/RR).P and TM7a/b-8.P (⌬NGGLQP), respectively, into HindIII-BstEII-digested pSPMDR1-P.
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ABCB1 p.Asp744Arg 9725920:73:7
status: NEW289 Effect of mutations D743R and D744R on TM7b ST activity and TM8 topology.
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ABCB1 p.Asp744Arg 9725920:289:30
status: NEW302 Moreover, each of these mutations (e.g., D743R/D744R, ⌬NGGLQP, and ⌬7b) also affected the stability and intracellular trafficking of full-length MDR1-Pgp protein in intact XOs (Bragin and Skach, unpublished observations), suggesting that structural features responsible for topological heterogeneity of MDR1-Pgp also contribute important information for protein maturation in cells.
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ABCB1 p.Asp744Arg 9725920:302:47
status: NEW