ABCMdb

PMID: 9725920

Moss K, Helm A, Lu Y, Bragin A, Skach WR
Coupled translocation events generate topological heterogeneity at the endoplasmic reticulum membrane.
Mol Biol Cell. 1998 Sep;9(9):2681-97., [PubMed]

Sentences

No. Mutations Sentence Comment

62 ABCB1 p.Asp744Arg ABCB1 p.Asp743Arg We then used the PCR overlap extension method to introduced mutations D743R/D744R. Login to comment 73 ABCB1 p.Asp744Arg ABCB1 p.Asp743Arg D743R/ D744R and ⌬NGGLQP mutations were subsequently engineered into pSPMDR1-P by ligating HindIII-BstEII fragments from plasmids TM7a/b-8(DD/RR).P and TM7a/b-8.P (⌬NGGLQP), respectively, into HindIII-BstEII-digested pSPMDR1-P. Login to comment 289 ABCB1 p.Asp744Arg ABCB1 p.Asp743Arg Effect of mutations D743R and D744R on TM7b ST activity and TM8 topology. Login to comment 302 ABCB1 p.Asp744Arg ABCB1 p.Asp743Arg Moreover, each of these mutations (e.g., D743R/D744R, ⌬NGGLQP, and ⌬7b) also affected the stability and intracellular trafficking of full-length MDR1-Pgp protein in intact XOs (Bragin and Skach, unpublished observations), suggesting that structural features responsible for topological heterogeneity of MDR1-Pgp also contribute important information for protein maturation in cells. Login to comment