ABCB1 p.Asn21Val
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PMID: 19557637
[PubMed]
Pop IV et al: "Targeting mammalian target of rapamycin to both downregulate and disable the P-glycoprotein pump in multidrug-resistant B-cell lymphoma cell lines."
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Comment
28
Materials and methods Cell lines The following MDR cell lines were used: two Burkitt lymphoma cell lines Namalwa 21 nM Vincristine (VCR) (N21V), and Raji 18 nM VCR (R18V), and the diffuse hystiocytic lymphoma, DHL-4/12 nM VCR (D12V), as well as the standard MDR cells, Namalwa/MDR1, which were a gift from Dr. R. O`Connor at ImmunoGen (Boston, MA).
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ABCB1 p.Asn21Val 19557637:28:138
status: NEW30 The MDR variants of Raji, DHL-4, and Namalwa, were generated by drug-selection in our laboratory and were named by the parental cells name and the final concentration of VCR to which they were resistant (e.g., N21V, R18V, and DHL-4/ 12V) [7].
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ABCB1 p.Asn21Val 19557637:30:210
status: NEW70 Equal amounts of protein from each cells lysate were loaded and gels were scanned and analyzed quantitatively to compare the amount of P-gp expressed by each MDR cell line: Namalwa/MDR1; Namalwa 21 nM VCR (N21V); Raji 18 nM VCR (R18V); and DHL-4/12 nM VCR (DHL-4/12V).
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ABCB1 p.Asn21Val 19557637:70:206
status: NEW77 As shown in the figure, Namalwa/MDR1 and N21V cells reached their peak of growth inhibition after 48 h of incubation in the presence of 0.55 mM rapamycin, whereas R18V continued to be inhibited for up to 72 h, and DHL-4/12V was inhibited up to 96 h.
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ABCB1 p.Asn21Val 19557637:77:41
status: NEW82 The cells express variable amounts of P-gp: Namalwa/MDR1 4 R18V 4 D12V 4 N21V.
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ABCB1 p.Asn21Val 19557637:82:73
status: NEW93 As shown in the top line of Figure 1, and in Figure 5, the expression of P-gp is down-regulated in rapamycin-treated Namalwa/MDR1 and N21V cells.
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ABCB1 p.Asn21Val 19557637:93:133
status: NEW94 The effect of rapamycin on the growth of Namalwa 21 nM VCR cells in vivo In these experiments, N21V cells were injected intravenously into SCID mice.
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ABCB1 p.Asn21Val 19557637:94:95
status: NEW101 ); Namalwa 21 nM VCR (N21V) (); Raji 18 nM VCR (R18V) (!
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ABCB1 p.Asn21Val 19557637:101:22
status: NEW114 As expected, the SCID mice with N21V tumors did not respond to treatment with VCR but did respond to rapamycin.
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ABCB1 p.Asn21Val 19557637:114:32
status: NEW118 Discussion We have previously described the generation of three drug-resistant lymphoma cell lines (N21V, R18V, and DHL-4/12V) by drug-selection and these cells expressed P-gp and effluxed rhodamine, suggesting that the P-gp pump was active [7].
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ABCB1 p.Asn21Val 19557637:118:100
status: NEW157 Namalwa/MDR1 and N21V cells treated as shown in the legend of Figure 1 were analyzed by FACS for P-gp expression after 48 h incubation at 378C using a UIC2-shift assay.
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ABCB1 p.Asn21Val 19557637:157:17
status: NEW160 The graph shows the results obtained using Namalwa/MDR1 and Namalwa 21 nM VCR (N21V) cells.
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ABCB1 p.Asn21Val 19557637:160:79
status: NEW163 Survival of SCID mice xenografted with the MDR tumor N21V cells and treated with rapamycin + VCR.
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ABCB1 p.Asn21Val 19557637:163:53
status: NEW
PMID: 18357372
[PubMed]
Pop I et al: "Generation of multidrug resistant lymphoma cell lines stably expressing P-glycoprotein."
No.
Sentence
Comment
29
The cells were named by the parental cell names and the final concentration of VCR to which they were resistant (e.g. N21V, R18V and DHL-4/12V).
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ABCB1 p.Asn21Val 18357372:29:118
status: NEW81 However, 80-90% of drug-selected cells (N21V, R18V and DHL-4/12V) were P-gp+ and the expression of P-gp remained unchanged when the cells were grown in complete RPMI media without VCR.
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ABCB1 p.Asn21Val 18357372:81:40
status: NEW102 As shown in Fig. 4, Namalwa/ MDR1 and N21V cells expressed comparable amounts of P-gp, whereas the R18V cells expressed more P-gp and the DHL-4/12V less than Namalwa/MDR1 for the same amount of protein loaded, which is consistent with the FACS analysis (Table II).
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ABCB1 p.Asn21Val 18357372:102:38
status: NEW109 The phenotype of MDR vs. parental cells.a ------------------------------------------------- Percent of positive cells Cell line CD19 CD20 CD22 P-gp ------------------------------------------------- Namalwa 95.7 83.5 95.5 7.1 NMDR1 92.7 90.9 91.7 90.0 N21V 93.3 90.4 93.3 91.9 Raji 92.0 98.1 91.9 1.8 R18V 92.0 97.3 94.3 85.7 DHL-4 77.8 99.7 76.4 4.1 DHL-4/12V 89.6 98.8 89.6 76.3 ------------------------------------------------- a FACS analysis was performed using saturating concentrations of HD37 and RFB4 and a FITC-GAMIg as a secondary antibody.
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ABCB1 p.Asn21Val 18357372:109:251
status: NEW113 N21V, R18V and Namalwa/MDR1 cells were inoculated into SCID mice and the tumor take was 100%.
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ABCB1 p.Asn21Val 18357372:113:0
status: NEW121 We chose to generate: i) N21V cells, as an alternative to Namalwa/MDR1 cells; ii) R18V cells since Raji cells express more CD20 than Namalwa cells; and iii) DHL-4/12V cells, which are derived from diffuse hystiocytic lymphoma cells, whereas the others are Burkitt's lymphomas.
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ABCB1 p.Asn21Val 18357372:121:25
status: NEW142 (A) Namalwa (ߦ), Namalwa/MDR1 (ߥ) and Namalwa 21 nM VCR (N21V) (t); (B) Raji (n) and Raji 12 nM VCR (R12V) (Þb;).
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ABCB1 p.Asn21Val 18357372:142:69
status: NEW158 The top histograms show the Namalwa 21 nM VCR (N21V) cells and the bottom histograms the Raji 18 nM VCR (R18V).
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ABCB1 p.Asn21Val 18357372:158:47
status: NEW164 The N21V and Namalwa/MDR1 cells contain a comparable amount of P-gp whereas R18V contain more and the DHL-4/12V cells contain less P-gp than the Namalwa/MDR1.
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ABCB1 p.Asn21Val 18357372:164:4
status: NEW175 WB analysis of P-gp overexpression in the new MDR cells (N21V, R18V and DHL-4/12V) and Namalwa/MDR1 vs. P-gp- parental cells.
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ABCB1 p.Asn21Val 18357372:175:57
status: NEW176 Equal amounts of protein from each cell lysate were loaded and the gels were scanned and analyzed quantitatively to compare the amount of P-gp expressed by each MDR cell line: (1) Namalwa, (2) Namalwa/MDR1, (3) Namalwa 21 nM VCR (N21V), (4) Raji, (5) Raji 18 nM VCR (R18V), (6) DHL-4 and (7) DHL-4/12 nM VCR (DHL-4/12V).
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ABCB1 p.Asn21Val 18357372:176:230
status: NEW178 Survival of SCID mice xenografted with parental tumors (Namalwa and Raji) or MDR tumors (Namalwa/MDR1, N21V and R18V) before and after treatment with VCR.
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ABCB1 p.Asn21Val 18357372:178:103
status: NEW183 (A) shows the survival of SCID mice xenografted with Namalwa cells &#b1; VCR (ߦ, control and ߥ, VCR); Namalwa/MDR1 &#b1; VCR (t, control and Ɗ, VCR) and N21V &#b1; VCR (ߦ, control and ߥ, VCR).
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ABCB1 p.Asn21Val 18357372:183:170
status: NEW