ABCMdb

PMID: 18357372

Pop I, Pop L, Vitetta ES, Ghetie MA
Generation of multidrug resistant lymphoma cell lines stably expressing P-glycoprotein.
Oncol Rep. 2008 Apr;19(4):889-95., [PubMed]

Sentences

No. Mutations Sentence Comment

29 ABCB1 p.Asn21Val The cells were named by the parental cell names and the final concentration of VCR to which they were resistant (e.g. N21V, R18V and DHL-4/12V). Login to comment 81 ABCB1 p.Asn21Val However, 80-90% of drug-selected cells (N21V, R18V and DHL-4/12V) were P-gp+ and the expression of P-gp remained unchanged when the cells were grown in complete RPMI media without VCR. Login to comment 102 ABCB1 p.Asn21Val As shown in Fig. 4, Namalwa/ MDR1 and N21V cells expressed comparable amounts of P-gp, whereas the R18V cells expressed more P-gp and the DHL-4/12V less than Namalwa/MDR1 for the same amount of protein loaded, which is consistent with the FACS analysis (Table II). Login to comment 109 ABCB1 p.Asn21Val The phenotype of MDR vs. parental cells.a ------------------------------------------------- Percent of positive cells Cell line CD19 CD20 CD22 P-gp ------------------------------------------------- Namalwa 95.7 83.5 95.5 7.1 NMDR1 92.7 90.9 91.7 90.0 N21V 93.3 90.4 93.3 91.9 Raji 92.0 98.1 91.9 1.8 R18V 92.0 97.3 94.3 85.7 DHL-4 77.8 99.7 76.4 4.1 DHL-4/12V 89.6 98.8 89.6 76.3 ------------------------------------------------- a FACS analysis was performed using saturating concentrations of HD37 and RFB4 and a FITC-GAMIg as a secondary antibody. Login to comment 113 ABCB1 p.Asn21Val N21V, R18V and Namalwa/MDR1 cells were inoculated into SCID mice and the tumor take was 100%. Login to comment 121 ABCB1 p.Asn21Val We chose to generate: i) N21V cells, as an alternative to Namalwa/MDR1 cells; ii) R18V cells since Raji cells express more CD20 than Namalwa cells; and iii) DHL-4/12V cells, which are derived from diffuse hystiocytic lymphoma cells, whereas the others are Burkitt's lymphomas. Login to comment 142 ABCB1 p.Asn21Val (A) Namalwa (ߦ), Namalwa/MDR1 (ߥ) and Namalwa 21 nM VCR (N21V) (t); (B) Raji (n) and Raji 12 nM VCR (R12V) (Þb;). Login to comment 158 ABCB1 p.Asn21Val The top histograms show the Namalwa 21 nM VCR (N21V) cells and the bottom histograms the Raji 18 nM VCR (R18V). Login to comment 164 ABCB1 p.Asn21Val The N21V and Namalwa/MDR1 cells contain a comparable amount of P-gp whereas R18V contain more and the DHL-4/12V cells contain less P-gp than the Namalwa/MDR1. Login to comment 175 ABCB1 p.Asn21Val WB analysis of P-gp overexpression in the new MDR cells (N21V, R18V and DHL-4/12V) and Namalwa/MDR1 vs. P-gp- parental cells. Login to comment 176 ABCB1 p.Asn21Val Equal amounts of protein from each cell lysate were loaded and the gels were scanned and analyzed quantitatively to compare the amount of P-gp expressed by each MDR cell line: (1) Namalwa, (2) Namalwa/MDR1, (3) Namalwa 21 nM VCR (N21V), (4) Raji, (5) Raji 18 nM VCR (R18V), (6) DHL-4 and (7) DHL-4/12 nM VCR (DHL-4/12V). Login to comment 178 ABCB1 p.Asn21Val Survival of SCID mice xenografted with parental tumors (Namalwa and Raji) or MDR tumors (Namalwa/MDR1, N21V and R18V) before and after treatment with VCR. Login to comment 183 ABCB1 p.Asn21Val (A) shows the survival of SCID mice xenografted with Namalwa cells &#b1; VCR (ߦ, control and ߥ, VCR); Namalwa/MDR1 &#b1; VCR (t, control and Ɗ, VCR) and N21V &#b1; VCR (ߦ, control and ߥ, VCR). Login to comment