ABCB1 p.Ala63Arg
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PMID: 18596043
[PubMed]
Loo TW et al: "Arginines in the first transmembrane segment promote maturation of a P-glycoprotein processing mutant by hydrogen bond interactions with tyrosines in transmembrane segment 11."
No.
Sentence
Comment
151
By contrast, 12 of the mutants (V53R, G54R, T55R, L56R, A57R, A58R, I59R, I60R, G62R, A63R, P66R, and F72R) showed little or no rescue in the presence of drug substrates.
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ABCB1 p.Ala63Arg 18596043:151:86
status: NEW165 TABLE 1 Effects of drug substrates on the maturation of TM1 arginine mutants containing the G251V mutation Mutation (G251V ؉) No drug Cyclosporin A Verapamil Vinblastine Rhodamine None - 111a,c 111 111 11 V52R -a,b 111 111 111 11 V53R 2 - - - - G64R 2 - - - - T55R 2 - - - - L56R 2 - - - - A57R 2 - - - - A58R 2 - - - - I59R 2 - - - - I60R 2 - - - - H61R - 111 1 1 1 G62R 2 - - - - A63R 2 - - - - G64R 1 111 1 1 11 L65R 11 111 11 11 11 P66R 2 1 - - - L67R - 111 111 111 11 M68R 111 111 111 111 111 M69R - 111 - 11 111 L70R - 111 111 111 11 V71R 1 111 111 111 11 F72R 2 1 1 1 1 a Change in the amount of mature (170 kDa) protein in the presence of drug substrate relative to that in the absence of drug substrate.
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ABCB1 p.Ala63Arg 18596043:165:388
status: NEW
PMID: 9287132
[PubMed]
Taguchi Y et al: "Amino acid substitutions in the first transmembrane domain (TM1) of P-glycoprotein that alter substrate specificity."
No.
Sentence
Comment
42
Cells transfected by He60 -> Arg, or Ala63 -> Arg cDNA were selected with stepwise increasing concentrations (10, 20, 40 ng/ml) of vinblastine and finally maintained in 30 ng/ml Vbl.
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ABCB1 p.Ala63Arg 9287132:42:37
status: NEW55 From cells transfected with the wild type and He60 -> Arg, His61 -> Arg, Ala63 -> Arg, Gly64 -> Arg, or Leu65 -> Arg mutant cDNA, both Vbl- and Col-resistant colonies were obtained (Fig. 1).
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ABCB1 p.Ala63Arg 9287132:55:73
status: NEW77 In contrast, the resistance profile of He60 -»Arg or Ala63 -> Arg mutant P-glycoprotein did not altered much from that of the wild type.
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ABCB1 p.Ala63Arg 9287132:77:58
status: NEW90 The substrate specificities of Gly64 -»Arg and Leu65 -> Arg mutant P-glycoproteins were quite different from that of the wild type, and similar to that of His61 -»Arg mutant, while He60 and Ala63 -> Arg mutant P-glycoproteins showed similar substrate specificities to that of the wild-type P-glycoprotein.
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ABCB1 p.Ala63Arg 9287132:90:200
status: NEW