ABCB1 p.Asn94Ala
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PMID: 18596043
[PubMed]
Loo TW et al: "Arginines in the first transmembrane segment promote maturation of a P-glycoprotein processing mutant by hydrogen bond interactions with tyrosines in transmembrane segment 11."
No.
Sentence
Comment
128
Mutants ⌬NBD2- P-gp, M68R/⌬NBD2-P-gp, and ⌬NBD2-P-gp lacking the three glycosylation sites (N91A, N94A, N99A) were expressed in HEK 293 cells, and whole cell SDS extracts were subjected to immunoblot analysis.
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ABCB1 p.Asn94Ala 18596043:128:119
status: NEW160 Whole cell extracts of HEK 293 cells expressing mutants ⌬NBD2-P-gp with no changes (None), the M68R mutation, or N91A, N94A and N99A changes to the glycosylation sites (Unglycos) were subjected to immunoblotanalysis.Thepositionsofmature,immature,andunglycosylated(Unglycos) forms of ⌬NBD2 P-gp are indicated.
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ABCB1 p.Asn94Ala 18596043:160:126
status: NEW
PMID: 9405384
[PubMed]
Loo TW et al: "Identification of residues in the drug-binding site of human P-glycoprotein using a thiol-reactive substrate."
No.
Sentence
Comment
65
B, whole cell extracts of HEK 293 cells expressing wild-type, Cys-less, glycosylation-deficient N91A/N94A/N99A and single Cys mutants that exhibited little or no verapamil-stimulated ATPase activity were subjected to immunoblot analysis as described under "Experimental Procedures."
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ABCB1 p.Asn94Ala 9405384:65:101
status: NEW90 This appeared to be a degradation product rather than a nonglycosylated product because it had a higher mobility than the glycosylation-deficient P-glycoprotein (N91A/N94A/N99A) (Fig. 2B, lanes 23 and 24).
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ABCB1 p.Asn94Ala 9405384:90:167
status: NEW
PMID: 9829963
[PubMed]
Loo TW et al: "Quality control by proteases in the endoplasmic reticulum. Removal of a protease-sensitive site enhances expression of human P-glycoprotein."
No.
Sentence
Comment
33
A glycosylation-deficient mutant was made by mutating the three consensus glycosylation sites (N91A,N94A,N99A).
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ABCB1 p.Asn94Ala 9829963:33:100
status: NEW71 The 130-kDa product was also observed when the glycosylation-deficient (N91A,N94A,N99A) mutant was expressed in the presence of proteasome inhibitors (Fig. 1, lanes 5 and 6).
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ABCB1 p.Asn94Ala 9829963:71:77
status: NEW93 HEK 293 cells were transfected with Cys-less (C-less), mutant G341C (in Cys-less background), or glycosylation-deficient (N91A,N94A,N99A; -Glycos.)
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ABCB1 p.Asn94Ala 9829963:93:127
status: NEW
PMID: 11361134
[PubMed]
Urbatsch IL et al: "Purification and characterization of N-glycosylation mutant mouse and human P-glycoproteins expressed in Pichia pastoris cells."
No.
Sentence
Comment
45
A BglII-BglII fragment from expression vector pMT21 containing mutations N91A, N94A, and N99A in the human MDR1 sequence (21) was transferred into pHIL-MDR1.4-His10(⌬BglII) (above) yielding pHIL- AAAMDR1.4-His10.
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ABCB1 p.Asn94Ala 11361134:45:79
status: NEW47 The N94A mutation generates a new BtsI site.
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ABCB1 p.Asn94Ala 11361134:47:4
status: NEW