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PMID: 11361134
Urbatsch IL, Wilke-Mounts S, Gimi K, Senior AE
Purification and characterization of N-glycosylation mutant mouse and human P-glycoproteins expressed in Pichia pastoris cells.
Arch Biochem Biophys. 2001 Apr 1;388(1):171-7.,
[PubMed]
Sentences
No.
Mutations
Sentence
Comment
44
ABCB1 p.Asn99Gln
X
ABCB1 p.Asn99Gln 11361134:44:4
status:
NEW
view ABCB1 p.Asn99Gln details
The
N99Q
mutation generates a new BciVI restriction site.
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45
ABCB1 p.Asn91Ala
X
ABCB1 p.Asn91Ala 11361134:45:73
status:
NEW
view ABCB1 p.Asn91Ala details
ABCB1 p.Asn94Ala
X
ABCB1 p.Asn94Ala 11361134:45:79
status:
NEW
view ABCB1 p.Asn94Ala details
ABCB1 p.Asn99Ala
X
ABCB1 p.Asn99Ala 11361134:45:89
status:
NEW
view ABCB1 p.Asn99Ala details
A BglII-BglII fragment from expression vector pMT21 containing mutations
N91A
,
N94A
, and
N99A
in the human MDR1 sequence (21) was transferred into pHIL-MDR1.4-His10(⌬BglII) (above) yielding pHIL- AAAMDR1.4-His10.
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47
ABCB1 p.Asn94Ala
X
ABCB1 p.Asn94Ala 11361134:47:4
status:
NEW
view ABCB1 p.Asn94Ala details
The
N94A
mutation generates a new BtsI site.
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