ABCB1 p.Arg580Cys
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PMID: 10581253
[PubMed]
Blott EJ et al: "Cysteine-scanning mutagenesis provides no evidence for the extracellular accessibility of the nucleotide-binding domains of the multidrug resistance transporter P-glycoprotein."
No.
Sentence
Comment
39
Four SC mutants (P549C, V569C, K578C and R580C) were expressed at significantly lower levels than wild-type P-gp, but use of an alternative transfection reagent (lipofectamine) allowed sufficient expression for further analysis.
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ABCB1 p.Arg580Cys 10581253:39:41
status: NEW59 Cells were transiently transfected with the relevant cDNA, using lipofectin or lipofectamine (for SC mutants P549C, V569C, K578C and R580C), and 75 µg of whole cell lysate (150 µg for K578C and R580C) was added per lane.
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ABCB1 p.Arg580Cys 10581253:59:133
status: NEWX
ABCB1 p.Arg580Cys 10581253:59:204
status: NEW
PMID: 12820887
[PubMed]
Gabriel MP et al: "Communication between the nucleotide binding domains of P-glycoprotein occurs via conformational changes that involve residue 508."
No.
Sentence
Comment
148
No recombinant virus could be obtained for R580C despite several independent cotransfection experiments.
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ABCB1 p.Arg580Cys 12820887:148:43
status: NEW