ABCC7 p.Gln1280Cys
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 18305154
[PubMed]
Serohijos AW et al: "Phenylalanine-508 mediates a cytoplasmic-membrane domain contact in the CFTR 3D structure crucial to assembly and channel function."
No.
Sentence
Comment
72
Cross-linking of C276/Q1280C and C276/K1284C confirms interaction of CL2 and NBD2.
X
ABCC7 p.Gln1280Cys 18305154:72:22
status: NEW101 (Right, Bottom two) Controls showing the lack of effects of cross-linking of the Cys-less construct and individually expressed and mixed single cysteine constructs (labeled 276C ϩ Q1280C) under the same conditions (see also SI Fig. 8).
X
ABCC7 p.Gln1280Cys 18305154:101:186
status: NEW103 (Left) There is greater propensity for disulfide bond formation at the CL2/NBD2 interface (276C/Q1280C) than at the CL4/NBD1 interface.
X
ABCC7 p.Gln1280Cys 18305154:103:96
status: NEW113 In fact, the Q1280C and K1284C substitutions in NBD2 of Cys-less CFTR containing the native Cys-276 residue in CL2 (illustrated in Fig. 3A, Top Right) enabled cross-linking by all MTS reagents tested.
X
ABCC7 p.Gln1280Cys 18305154:113:13
status: NEW130 (Middle) Cys-less CFTR with 276C/Q1280C (n ϭ 3).
X
ABCC7 p.Gln1280Cys 18305154:130:33
status: NEW
PMID: 20233947
[PubMed]
He L et al: "Restoration of domain folding and interdomain assembly by second-site suppressors of the DeltaF508 mutation in CFTR."
No.
Sentence
Comment
122
As shown in Fig. 3A, when ⌬F508 was introduced into Cys-less CFTR containing pairs 276C/Q1280C or 276C/K1284C at the NBD2/CL2 interface, no mature CFTR was detected, and neither M3M nor M8M (200 M) caused cross-linking of the immature band.
X
ABCC7 p.Gln1280Cys 20233947:122:95
status: NEW125 As shown in Fig. 3A, ⌬F508-CFTR maturation was restored as was the cross-linking of the Cys pairs 276C/Q1280C and 276C/ K1284C at NBD2/CL2, as indicated by the slower migration rate of the mature bands in SDS-PAGE in samples treated with M3M and M8M (18).
X
ABCC7 p.Gln1280Cys 20233947:125:110
status: NEW
PMID: 20561529
[PubMed]
Aleksandrov AA et al: "Regulatory insertion removal restores maturation, stability and function of DeltaF508 CFTR."
No.
Sentence
Comment
154
HEK 293 cells were transiently transfected with Cys-less ΔF508 CFTR with Cys pairs introduced at the CL2-NBD2 (C276-Q1280C) or CL4-NBD1 (V510C-G1069C) interfaces in the absence or presence of ΔRI.
X
ABCC7 p.Gln1280Cys 20561529:154:122
status: NEW
PMID: 15581632
[PubMed]
Buyse F et al: "Mistargeted MRPdeltaF728 mutant is rescued by intracellular GSH."
No.
Sentence
Comment
154
HEK 293 cells were transiently transfected with Cys-less ΔF508 CFTR with Cys pairs introduced at the CL2-NBD2 (C276-Q1280C) or CL4- NBD1 (V510C-G1069C) interfaces in the absence or presence of ΔRI.
X
ABCC7 p.Gln1280Cys 15581632:154:122
status: NEW
PMID: 23104983
[PubMed]
He L et al: "Correctors of DeltaF508 CFTR restore global conformational maturation without thermally stabilizing the mutant protein."
No.
Sentence
Comment
126
HEK293 cells were transiently transfected with Cys-less CFTR or Cys-less èc;F508 CFTR with Cys pairs 276C/Q1280C or V510C/G1069C, introduced at NBD2/CL2 and NBD1/CL4 interfaces, respectively (13).
X
ABCC7 p.Gln1280Cys 23104983:126:110
status: NEW