ABCC7 p.Asn900Gln
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PMID: 15923638
[PubMed]
Farinha CM et al: "Most F508del-CFTR is targeted to degradation at an early folding checkpoint and independently of calnexin."
No.
Sentence
Comment
208
As the N-glyconjugate is attached to asparagine residues 894 and 900 of CFTR, we replaced both these residues by either glutamine (N894Q and N900Q) or alanine (N894A and N900A) residues and studied the CFTR turnover of the resulting proteins on wt and F508del backgrounds when stably expressed in BHK cells as above.
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ABCC7 p.Asn900Gln 15923638:208:141
status: NEW219 BHK cells stably expressing wt CFTR (A, lanes 1 to 5), N894A-N900A (WAA) (A, lanes 6 to 10), N894Q-N900Q (WQQ) (A, lanes 11 to 15), F508del-CFTR (B, lanes 1 to 5), F508del-N894A-N900A (FAA) (B, lanes 6 to 10), or F508del-N894Q-N900Q CFTR (FQQ) (B, lanes 11 to 15) were pulse-labeled and chased as before (Fig. 2) for 0 h (lanes 1, 6, and 11), 0.5 h (lanes 2, 7, and 12), 1 h (lanes 3, 8, and 13), 2 h (lanes 4, 9, and 14), and 3 h (lanes 5, 10, and 15).
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ABCC7 p.Asn900Gln 15923638:219:99
status: NEWX
ABCC7 p.Asn900Gln 15923638:219:227
status: NEW284 Asparagine residues at positions 894 and 900 were replaced by either two alanines (N894A and N900A) or two glutamines (N894Q and N900Q).
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ABCC7 p.Asn900Gln 15923638:284:129
status: NEW