ABCC7 p.Cys225Ala
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PMID: 11748233
[PubMed]
Therien AG et al: "Interhelical packing in detergent micelles. Folding of a cystic fibrosis transmembrane conductance regulator construct."
No.
Sentence
Comment
20
It should be noted that Cys-225 was mutated to Ala, and two cysteines (underlined) * This work was supported by grants (to C. M. D.) from the Canadian Cystic Fibrosis Foundation, the Canadian Institutes of Health Research, and the NIDDK, National Institutes of Health.
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ABCC7 p.Cys225Ala 11748233:20:24
status: NEW44 Because pyrene is available commercially as a cysteine-reactive molecule (pyrene iodoacetamide), we mutated TM 3-4 in the following ways (see also Ref. 21): (i) Cys-225 was mutated to Ala to remove any other possible reactive sites, and (ii) Cys residues were inserted at the ends of TM segments 3 and 4.
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ABCC7 p.Cys225Ala 11748233:44:161
status: NEW
PMID: 11888281
[PubMed]
Partridge AW et al: "Polar residues in membrane domains of proteins: molecular basis for helix-helix association in a mutant CFTR transmembrane segment."
No.
Sentence
Comment
107
Spectra are TM4-VD and TM4-VD(C225A).
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ABCC7 p.Cys225Ala 11888281:107:30
status: NEW
PMID: 15776432
[PubMed]
Clain J et al: "Misprocessing of the CFTR protein leads to mild cystic fibrosis phenotype."
No.
Sentence
Comment
201
To test this possibility, these residues were replaced one at a time by Ala (p.T122A, p.C128A, p.S135A, p.C225A, and p.Q237A) together with the p.L206W mutation, and the processing at steady-state was examined.
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ABCC7 p.Cys225Ala 15776432:201:106
status: NEW
PMID: 17516627
[PubMed]
Wehbi H et al: "Role of the extracellular loop in the folding of a CFTR transmembrane helical hairpin."
No.
Sentence
Comment
39
The resulting construct, designated TM3/4 WT in this work, contains a C225A replacement designed to prevent disulfide bond formation between different helical hairpin molecules, has a 32-residue N-terminal fusion with an S-tag epitope used for Western blot detection (sequence GSGMKETAAAKFERQHMDSP- DLGTDDDDKAM), and has an 8-residue C-terminal fusion with the hexahistidine tag used for purification by affinity chromatography (sequence LEHHHHHH).
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ABCC7 p.Cys225Ala 17516627:39:70
status: NEW