ABCMdb

PMID: 11748233

Therien AG, Deber CM
Interhelical packing in detergent micelles. Folding of a cystic fibrosis transmembrane conductance regulator construct.
J Biol Chem. 2002 Feb 22;277(8):6067-72. Epub 2001 Dec 17., 2002-02-22 [PubMed]

Sentences

No. Mutations Sentence Comment

20 ABCC7 p.Cys225Ala It should be noted that Cys-225 was mutated to Ala, and two cysteines (underlined) * This work was supported by grants (to C. M. D.) from the Canadian Cystic Fibrosis Foundation, the Canadian Institutes of Health Research, and the NIDDK, National Institutes of Health. Login to comment 44 ABCC7 p.Cys225Ala Because pyrene is available commercially as a cysteine-reactive molecule (pyrene iodoacetamide), we mutated TM 3-4 in the following ways (see also Ref. 21): (i) Cys-225 was mutated to Ala to remove any other possible reactive sites, and (ii) Cys residues were inserted at the ends of TM segments 3 and 4. Login to comment 136 ABCC7 p.Val232Asp dition, since TM 3-4 constructs represent a segment of the CFTR protein, experiments become feasible which test the effects of various cystic fibrosis phenotypic mutations on interhelical packing of TMs 3 and 4, as described for the mutant V232D (21). Login to comment