ABCC1 p.Tyr324Trp
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PMID: 17980150
[PubMed]
DeGorter MK et al: "Molecular modeling of the human multidrug resistance protein 1 (MRP1/ABCC1)."
No.
Sentence
Comment
46
To create the single mutants Y324A, Y324F and Y324W, the QuikChangeä site-directed mutagenesis kit (Stratagene Inc., La Jolla, CA), was used.
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ABCC1 p.Tyr324Trp 17980150:46:46
status: NEW48 The mutations were introduced using the following sense primers (mutated residues are underlined, silent mutations to introduce restriction sites are italicized, and restriction enzymes used are indicated in parentheses): Y324A, 50 -GAAGAAGCT CATGAGGAATGCGGGCCCAAAGGTC-30 (BsmI); Y324F, 50 -GCT CATGAGGAAGAACGGCCCAAAGGTCTTG-30 (ApaI); Y324W, 50 - GAAGAAGCTCATCAGGAACCAGGGCCCAAAGGTC-30 (BspHI).
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ABCC1 p.Tyr324Trp 17980150:48:335
status: NEW