ABCMdb

PMID: 17980150

DeGorter MK, Conseil G, Deeley RG, Campbell RL, Cole SP
Molecular modeling of the human multidrug resistance protein 1 (MRP1/ABCC1).
Biochem Biophys Res Commun. 2008 Jan 4;365(1):29-34. Epub 2007 Oct 31., 2008-01-04 [PubMed]

Sentences

No. Mutations Sentence Comment

34 ABCC1 p.Tyr324Ala Consequently, we have tested the functional importance of this residue by using site-directed mutagenesis to replace Tyr324 with Ala, Phe and Trp, and characterizing the transport activity of the resulting mutants. Login to comment 46 ABCC1 p.Tyr324Ala ABCC1 p.Tyr324Trp ABCC1 p.Tyr324Phe To create the single mutants Y324A, Y324F and Y324W, the QuikChangeä site-directed mutagenesis kit (Stratagene Inc., La Jolla, CA), was used. Login to comment 48 ABCC1 p.Tyr324Ala ABCC1 p.Tyr324Trp ABCC1 p.Tyr324Phe The mutations were introduced using the following sense primers (mutated residues are underlined, silent mutations to introduce restriction sites are italicized, and restriction enzymes used are indicated in parentheses): Y324A, 50 -GAAGAAGCT CATGAGGAATGCGGGCCCAAAGGTC-30 (BsmI); Y324F, 50 -GCT CATGAGGAAGAACGGCCCAAAGGTCTTG-30 (ApaI); Y324W, 50 - GAAGAAGCTCATCAGGAACCAGGGCCCAAAGGTC-30 (BspHI). Login to comment 105 ABCC1 p.Tyr324Phe In the case of Y324F, the transport of all four organic anion substrates tested was increased 1.5to 1.8-fold relative to wild-type MRP1. Login to comment