ABCC1 p.Lys347Leu
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 15155831
[PubMed]
Haimeur A et al: "Mutations of charged amino acids in or near the transmembrane helices of the second membrane spanning domain differentially affect the substrate specificity and transport activity of the multidrug resistance protein MRP1 (ABCC1)."
No.
Sentence
Comment
48
The sequences of the individual sense strands (with the corresponding amino acid changes indicated in parentheses, the altered codons underlined, and silent mutations introducing new restriction sites italicized) were as follows: K(D)332K, 5Ј-G AGC TTC TTC TTC AAG GCC ATC CAC GAC CTG-3Ј; K332R, 5Ј-G AGC TTC TTC TTC AGG GCC ATC CAC GAC CTG-3Ј; D(K)336E, 5Ј-C AAG GCC ATC CAC GAG CTC ATG ATG TTT TCC-3Ј; D336K, 5Ј-C AAG GCC ATC CAC AAG CTT ATG ATG TTT TCC-3Ј; K332D/D336K, 5Ј-GC TTC TTC TTC GAC GCC ATC CAC AAA CTG ATG ATG-3Ј; K319D, 5Ј-G TTT AAG GTG TTA TAC GAC ACG TTT GGG CCC-3Ј; K347D, 5Ј-GGG CCG CAG ATA TTA GAC TTG CTC ATC AAG-3Ј; K347L, 5Ј-GGG CCG CAA ATC TTA CTT TTG CTC ATC AAG-3Ј; D360K, 5Ј-GAC ACG AAG GCG CCA AAG TGG CAG GGC TAC-3Ј; R394D, 5Ј-C GTC AGT GGC ATG GAG ATC AAG ACC GCT GTC-3Ј; R394I, 5Ј-C GTC AGT GGC ATG ATC ATC AAG ACC GCT GTC-3Ј; K396E, 5Ј-GT GGC ATG AGG ATC GAG ACC GCT GTC ATT GGG-3Ј; K396I, 5Ј-GT GGC ATG AGG ATC ATC ACC GCT GTC ATT GGG-3Ј; K(E)396R, 5Ј-GGC ATG AGG ATC AGG ACC GCT GTC ATT GGG GC-3Ј; D430K, 5Ј-C AAC CTC ATG TCT GTG AAG GCT CAG AGG Fig. 1.
X
ABCC1 p.Lys347Leu 15155831:48:721
status: NEW136 Lys319 was replaced with Asp (K319D), and Lys347 was replaced with Asp (K347D) as well as Leu (K347L).
X
ABCC1 p.Lys347Leu 15155831:136:95
status: NEW138 Thus the ability of the K319D, K347D, and K347L mutants to transport GSH was reduced by approximately 50% (Fig. 4C), whereas the transport of four other substrates (LTC4, E217betaG, E13SO4 and MTX) by these mutants remained comparable with wild-type MRP1 (Table 2).
X
ABCC1 p.Lys347Leu 15155831:138:42
status: NEW177 C, apigenin-stimulated [3 H]GSH uptake at 20 min by wild-type (WT) MRP1 (f), mutants K319D, K347D, and K347L (u), and empty pcDNA3.1(-) vector control (Ⅺ).
X
ABCC1 p.Lys347Leu 15155831:177:103
status: NEW