ABCC1 p.Asp281Gly
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PMID: 11483599
[PubMed]
Sampath J et al: "Mutant p53 cooperates with ETS and selectively up-regulates human MDR1 not MRP1."
No.
Sentence
Comment
35
QuickChange site-directed mutagenesis (Stratagene, La Jolla, CA) was performed on the CMV-p53 plasmid with two oligonucleotide primers designed to change amino acid 281 from aspartate to glycine, and plasmid DNA was sequenced to identify the clones with the desired aspartate to glycine mutation.
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ABCC1 p.Asp281Gly 11483599:35:165
status: NEW52 The hot-spot MT p53 expression vectors have been described previously (V143A, R175H, R248W, R273H, and D281G) (13) and were provided kindly by Dr. Arnold J. Levine (Rockefeller University, New York).
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ABCC1 p.Asp281Gly 11483599:52:103
status: NEW98 Because it is unknown if p53-mediated repression of the human MDR1 promoter (7, 24) and its activation by MT p53 are mediated by distinct cis-elements, we used a series of MDR1 promoter deletion constructs to assess both repression by wt p53 and activation by the MT p53 containing an aspartate to glycine at amino acid 281 in p53 (p53-281).
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ABCC1 p.Asp281Gly 11483599:98:285
status: NEW235 For instance, heat shock 70 binds p53- 143A and R175H, but not R248W, R273H, or D281G (14, 36).
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ABCC1 p.Asp281Gly 11483599:235:80
status: NEW
PMID: 12647018
[PubMed]
Tsang WP et al: "Modulation of multidrug resistance-associated protein 1 (MRP1) by p53 mutant in Saos-2 cells."
No.
Sentence
Comment
27
Human osteosarcoma Saos-2 cells, a p53-null cell line, were transfected with five different p53 mutants (V143A, R175H, D248W, R273H and D281G).
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ABCC1 p.Asp281Gly 12647018:27:136
status: NEW
PMID: 9865734
[PubMed]
Wang Q et al: "Transcriptional suppression of multidrug resistance-associated protein (MRP) gene expression by wild-type p53."
No.
Sentence
Comment
76
Other vectors expressing a mutant human p53 were similarly con structed: (a) p53-175 is a mutant human p53 cDNA expression plasmid harboring a substitution from arginine to histidine at aa 175: (b) p53-281 has a mutation from aspartic acid to glycine at aa 281; and (c) p53-22/23 has mutations at aa 22 and aa 23.
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ABCC1 p.Asp281Gly 9865734:76:226
status: NEW