PMID: 11483599

Sampath J, Sun D, Kidd VJ, Grenet J, Gandhi A, Shapiro LH, Wang Q, Zambetti GP, Schuetz JD
Mutant p53 cooperates with ETS and selectively up-regulates human MDR1 not MRP1.
J Biol Chem. 2001 Oct 19;276(42):39359-67. Epub 2001 Aug 1., 2001-10-19 [PubMed]
Sentences
No. Mutations Sentence Comment
35 ABCC1 p.Asp281Gly
X
ABCC1 p.Asp281Gly 11483599:35:165
status: NEW
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 QuickChange site-directed mutagenesis (Stratagene, La Jolla, CA) was performed on the CMV-p53 plasmid with two oligonucleotide primers designed to change amino acid 281 from aspartate to glycine, and plasmid DNA was sequenced to identify the clones with the desired aspartate to glycine mutation. Login to comment 52 ABCC1 p.Asp281Gly
X
ABCC1 p.Asp281Gly 11483599:52:103
status: NEW
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 The hot-spot MT p53 expression vectors have been described previously (V143A, R175H, R248W, R273H, and D281G) (13) and were provided kindly by Dr. Arnold J. Levine (Rockefeller University, New York). Login to comment 98 ABCC1 p.Asp281Gly
X
ABCC1 p.Asp281Gly 11483599:98:285
status: NEW
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 Because it is unknown if p53-mediated repression of the human MDR1 promoter (7, 24) and its activation by MT p53 are mediated by distinct cis-elements, we used a series of MDR1 promoter deletion constructs to assess both repression by wt p53 and activation by the MT p53 containing an aspartate to glycine at amino acid 281 in p53 (p53-281). Login to comment 235 ABCC1 p.Asp281Gly
X
ABCC1 p.Asp281Gly 11483599:235:80
status: NEW
view ABCC1 p.Asp281Gly details
 For instance, heat shock 70 binds p53- 143A and R175H, but not R248W, R273H, or D281G (14, 36). Login to comment