ABCG8 p.Gly216Asp
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PMID: 16352607
[PubMed]
Zhang DW et al: "Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8."
No.
Sentence
Comment
179
We therefore substituted aspartate for glycine in the signature motif of G5 and G8 (G5-G197D and G8-G216D) to investigate the possible role of this motif in protein function.
X
ABCG8 p.Gly216Asp 16352607:179:100
status: VERIFIED183 Thus, G5-G197D and G8-G216D did not affect significantly the ATP labeling and ADP trapping in G5 and G8.
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ABCG8 p.Gly216Asp 16352607:183:22
status: VERIFIED185 In contrast to these results, co-expression of G8-G216D with wild-type G5 did not result in any increase in biliary cholesterol level and produced only a modest increase in the level of biliary plant sterols (Fig. 6B).
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ABCG8 p.Gly216Asp 16352607:185:50
status: VERIFIED
PMID: 16867993
[PubMed]
Wang J et al: "Sterol transfer by ABCG5 and ABCG8: in vitro assay and reconstitution."
No.
Sentence
Comment
37
Mutations predicted to inactivate the ATPases were introduced into the Walker A motif of G5 (K93M) and into the signature motif of G8 (G216D) using the QuickChange site-directed mutagenesis kit (Stratagene) (13, 14).
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ABCG8 p.Gly216Asp 16867993:37:135
status: VERIFIED108 A missense mutation in a highly conserved residue in the signature motif was introduced into G8 (G216D); substitution of the corresponding residue in two other ABC transporters impairs the ATPase and transport activity of both transporters (14, 23).
X
ABCG8 p.Gly216Asp 16867993:108:97
status: VERIFIED138 Recombinant wild type and mutant G5 (K93M) and G8 (G216D) were expressed in Sf9 cells.
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ABCG8 p.Gly216Asp 16867993:138:51
status: VERIFIED38 Mutations predicted to inactivate the ATPases were introduced into the Walker A motif of G5 (K93M) and into the signature motif of G8 (G216D) using the QuickChange site-directed mutagenesis kit (Stratagene) (13, 14).
X
ABCG8 p.Gly216Asp 16867993:38:135
status: NEW109 A missense mutation in a highly conserved residue in the signature motif was introduced into G8 (G216D); substitution of the corresponding residue in two other ABC transporters impairs the ATPase and transport activity of both transporters (14, 23).
X
ABCG8 p.Gly216Asp 16867993:109:97
status: NEW139 Recombinant wild type and mutant G5 (K93M) and G8 (G216D) were expressed in Sf9 cells.
X
ABCG8 p.Gly216Asp 16867993:139:51
status: NEW39 Mutations predicted to inactivate the ATPases were introduced into the Walker A motif of G5 (K93M) and into the signature motif of G8 (G216D) using the QuickChange site-directed mutagenesis kit (Stratagene) (13, 14).
X
ABCG8 p.Gly216Asp 16867993:39:135
status: NEW110 A missense mutation in a highly conserved residue in the signature motif was introduced into G8 (G216D); substitution of the corresponding residue in two other ABC transporters impairs the ATPase and transport activity of both transporters (14, 23).
X
ABCG8 p.Gly216Asp 16867993:110:97
status: NEW140 Recombinant wild type and mutant G5 (K93M) and G8 (G216D) were expressed in Sf9 cells.
X
ABCG8 p.Gly216Asp 16867993:140:51
status: NEW
PMID: 18402465
[PubMed]
Wang J et al: "Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8."
No.
Sentence
Comment
77
The knockout mice were infected with adenoviruses (5 × 1012 particles/kg injected into the tail vein) expressing either wild-type G5 and G8 or mutant forms of the protein that were previously demonstrated to lack ATPase activity: G5 (K93M) and G8 (G216D) (12).
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ABCG8 p.Gly216Asp 18402465:77:254
status: VERIFIED