ABCMdb

PMID: 16867993

Wang J, Sun F, Zhang DW, Ma Y, Xu F, Belani JD, Cohen JC, Hobbs HH, Xie XS
Sterol transfer by ABCG5 and ABCG8: in vitro assay and reconstitution.
J Biol Chem. 2006 Sep 22;281(38):27894-904. Epub 2006 Jul 25., 2006-09-22 [PubMed]

Sentences

No. Mutations Sentence Comment

37 ABCG8 p.Gly216Asp Mutations predicted to inactivate the ATPases were introduced into the Walker A motif of G5 (K93M) and into the signature motif of G8 (G216D) using the QuickChange site-directed mutagenesis kit (Stratagene) (13, 14). Login to comment 38 ABCG8 p.Gly216Asp Mutations predicted to inactivate the ATPases were introduced into the Walker A motif of G5 (K93M) and into the signature motif of G8 (G216D) using the QuickChange site-directed mutagenesis kit (Stratagene) (13, 14). Login to comment 39 ABCG8 p.Gly216Asp Mutations predicted to inactivate the ATPases were introduced into the Walker A motif of G5 (K93M) and into the signature motif of G8 (G216D) using the QuickChange site-directed mutagenesis kit (Stratagene) (13, 14). Login to comment 108 ABCG8 p.Gly216Asp A missense mutation in a highly conserved residue in the signature motif was introduced into G8 (G216D); substitution of the corresponding residue in two other ABC transporters impairs the ATPase and transport activity of both transporters (14, 23). Login to comment 109 ABCG8 p.Gly216Asp A missense mutation in a highly conserved residue in the signature motif was introduced into G8 (G216D); substitution of the corresponding residue in two other ABC transporters impairs the ATPase and transport activity of both transporters (14, 23). Login to comment 110 ABCG8 p.Gly216Asp A missense mutation in a highly conserved residue in the signature motif was introduced into G8 (G216D); substitution of the corresponding residue in two other ABC transporters impairs the ATPase and transport activity of both transporters (14, 23). Login to comment 138 ABCG8 p.Gly216Asp Recombinant wild type and mutant G5 (K93M) and G8 (G216D) were expressed in Sf9 cells. Login to comment 139 ABCG8 p.Gly216Asp Recombinant wild type and mutant G5 (K93M) and G8 (G216D) were expressed in Sf9 cells. Login to comment 140 ABCG8 p.Gly216Asp Recombinant wild type and mutant G5 (K93M) and G8 (G216D) were expressed in Sf9 cells. Login to comment