ABCG2 p.Cys438Ala
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PMID: 18430864
[PubMed]
Liu Y et al: "Effect of cysteine mutagenesis on the function and disulfide bond formation of human ABCG2."
No.
Sentence
Comment
111
Mutation of one or two of these residues (I2-CL, C284A, and C374A) did not signifi- TABLE 1 Primers used for construction of cysless mutants Mutations Primer Sequence RESa C43A TTTCATAACATTGCCTATCGAGTAAAACTGAAG BsrDI C55A GCTTTCTACCTGCACGAAAACCAGTTGAG BsgI C119A GCCAATTTCAAAGCGAATTCAGGTTACGTGG EcoRI C284A GAATCAGCTGGATATCACGCTGAGGCCTATAATAAC EcoRV C374A ACACCACCTCCTTCGCTCATCAACTCAGATG None C438A CTGACGACCAACCAAGCTTTCAGCAGTGTTTC HindIII C491A TATATTTACCGCTATAGTATACTTCATGTTAGG AccI C544A CTTCTCATGACGATCGCTTTTGTGTTTATGATG PvuI C592A GGACAAAACTTCGCCCCGGGACTCAATGCAA SmaI C603A/C608A AGGAAACAATCCTGCTAACTATGCAACAGCTACTGGCGAAGAATATTT -NspI C635A CACGTGGCCTTGGCTGCAATGATTGTTATTTTC BsrDI a Restriction (RES) enzyme digestion sites engineered in the primer for the convenience of detection.
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ABCG2 p.Cys438Ala 18430864:111:393
status: VERIFIED114 I3-CL with C284A, C374A, and C438A mutations also lost most of its ability to transport another substrate, Hoechst 33342 (Fig. 5D).
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ABCG2 p.Cys438Ala 18430864:114:29
status: VERIFIED150 C, relative activity of mutant ABCG2R482G I2-CL, C284A, I3-CL, and C374A compared with wild-type and ABCG2R482G as determined using flow cytometry for mitoxantrone (MX) accumulation in Sf9 cells. Data shown are from three independent experiments with S.D. D, effect of C284A, C374A, and C438A mutations (construct I3-CL) on efflux of Hoechst 33342 (Hoechst) as determined using flow cytometry.
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ABCG2 p.Cys438Ala 18430864:150:287
status: VERIFIED171 Mutation of Cys438 to alanine along with Cys374 (construct I2-CL) did not affect ABCG2R482G activity.
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ABCG2 p.Cys438Ala 18430864:171:12
status: VERIFIED172 C438A mutation alone also did not affect ABCG2R482G function (Kage et al., 2005).
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ABCG2 p.Cys438Ala 18430864:172:0
status: VERIFIED