ABCG2 p.Asn418Ala
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PMID: 15835752
[PubMed]
Takada T et al: "Characterization of polarized expression of point- or deletion-mutated human BCRP/ABCG2 in LLC-PK1 cells."
No.
Sentence
Comment
36
Using the site-directed mutagenesis technique, several mutants of BCRP (N418A, N596A, N418A&N596A BCRP and C592A, C603A&C608A, C592A&C603A&C608A BCRP) were constructed on pcDNA3.1(+) vector.
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ABCG2 p.Asn418Ala 15835752:36:72
status: VERIFIED57 Western blot analysis revealed that the modification was on the latter asparagine (Fig. 3B); the N418A mutant was N-glycosylated, whereas the N596A and N418A&N596A mutants did not exhibit any alteration in molecular weight following PNGase F treatment.
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ABCG2 p.Asn418Ala 15835752:57:97
status: VERIFIED
PMID: 15875186
[PubMed]
Mohrmann K et al: "Absence of N-linked glycosylation does not affect plasma membrane localization of breast cancer resistance protein (BCRP/ABCG2)."
No.
Sentence
Comment
45
BCRP-N418A, BCRP-N557A and BCRP-N596A were made using pcDNA3/BCRP wild type (GenBank accession number AY017168; generously provided by Susan Bates) as template.
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ABCG2 p.Asn418Ala 15875186:45:5
status: VERIFIED46 BCRP-N418A/ N557A was made using overlap extension PCR with pGEM-T Easy/BCRP-N418A as template and BCRP-N418A/N557A/N596A was made with pGEM-T Easy/ BCRP-N418A/N557A as template again using overlap extension PCR.
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ABCG2 p.Asn418Ala 15875186:46:5
status: VERIFIEDX
ABCG2 p.Asn418Ala 15875186:46:77
status: VERIFIEDX
ABCG2 p.Asn418Ala 15875186:46:104
status: VERIFIEDX
ABCG2 p.Asn418Ala 15875186:46:154
status: VERIFIED105 Expression of BCRP-N418A resulted in detection of two BCRP bands at the same position as in wild-type BCRP transfectants, indicating that asparagine 418 is not significantly glycosylated.
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ABCG2 p.Asn418Ala 15875186:105:19
status: VERIFIED156 Empty vector pcDNA3 or pcDNA3 with BCRP or mutant BCRP, BCRP-N418A, BCRP-N557A and BCRP-N596A, was transiently transfected into CHO9 cells or MDCKII cells using FuGENE6.
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ABCG2 p.Asn418Ala 15875186:156:61
status: VERIFIED186 Annu Rev Cell Dev Biol 16:113-143 Fig. 4 Wild-type BCRP, BCRP-N418A and BCRP-N596A are localized at the plasma membrane.
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ABCG2 p.Asn418Ala 15875186:186:62
status: VERIFIED188 Empty vector pcDNA3 or pcDNA3 with BCRP or mutant BCRP, BCRP-N418A, BCRP-N557A and BCRP-N596A, was transiently transfected into CHO9 cells or MDCKII cells using FuGENE6.
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ABCG2 p.Asn418Ala 15875186:188:61
status: VERIFIED