ABCMdb

PMID: 15835752

Takada T, Suzuki H, Sugiyama Y
Characterization of polarized expression of point- or deletion-mutated human BCRP/ABCG2 in LLC-PK1 cells.
Pharm Res. 2005 Mar;22(3):458-64., [PubMed]

Sentences

No. Mutations Sentence Comment

36 ABCG2 p.Asn596Ala ABCG2 p.Asn418Ala ABCG2 p.Cys592Ala Using the site-directed mutagenesis technique, several mutants of BCRP (N418A, N596A, N418A&N596A BCRP and C592A, C603A&C608A, C592A&C603A&C608A BCRP) were constructed on pcDNA3.1(+) vector. Login to comment 57 ABCG2 p.Asn596Ala ABCG2 p.Asn418Ala Western blot analysis revealed that the modification was on the latter asparagine (Fig. 3B); the N418A mutant was N-glycosylated, whereas the N596A and N418A&N596A mutants did not exhibit any alteration in molecular weight following PNGase F treatment. Login to comment 61 ABCG2 p.Cys592Ala Results of immunohistochemical staining suggested the importance of disulfide bonds on apical localization; a part of C592A mutant product seemed to be located on apical membrane, whereas other mutants appeared intracellularly (Fig. 4C). Login to comment