ABCG1 p.Gly121Ala
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PMID: 15994327
[PubMed]
Vaughan AM et al: "ABCG1 redistributes cell cholesterol to domains removable by high density lipoprotein but not by lipid-depleted apolipoproteins."
No.
Sentence
Comment
47
Site-directed mutagenesis of an amino acid residue common to all ABC transporter nucleotide binding domains (NCBI conserved domains data base, cd00267.1) (28) within the Walker A motif (29) of ABCG1 (glycine121 to alanine, GenBankTM accession number NP_004906) was carried out using the QuikChange XL site-directed mutagenesis kit (Stratagene, CA).
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ABCG1 p.Gly121Ala 15994327:47:200
status: VERIFIED140 The G121A mutation almost completely abolished the ability of ABCG1 to redistribute cholesterol to oxidase- and ACAT-accessible pools and to promote cholesterol efflux to HDL (Fig. 5D).
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ABCG1 p.Gly121Ala 15994327:140:4
status: VERIFIED160 There was no significant decrease in cholesterol content during the HDL incubation in the mock-transfected cells or in the ABCG1 G121A transfectants (Fig. 6C).
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ABCG1 p.Gly121Ala 15994327:160:129
status: VERIFIED181 C, equal amounts of cell protein from BHK cells transfected with FLAG-tagged wild type ABCG1 (WT) or a NBD ABCG1 mutant (G121A) were resolved by SDS-PAGE in the presence or absence of beta-mercaptoethanol (BME), and ABCG1 was detected by immunoblot analysis using an anti-FLAG antibody.
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ABCG1 p.Gly121Ala 15994327:181:121
status: VERIFIED185 *, p Ͻ 0.03 versus mock- or ABCG1-G121A-transfected cells.
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ABCG1 p.Gly121Ala 15994327:185:40
status: VERIFIED186 E, cell-surface (biotinylated) FLAG-tagged ABCG1 and ABCG1 G121A were detected by a streptavidin blotting assay following immunoprecipitation with an anti-FLAG antibody.
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ABCG1 p.Gly121Ala 15994327:186:59
status: VERIFIED
PMID: 16702602
[PubMed]
Kobayashi A et al: "Efflux of sphingomyelin, cholesterol, and phosphatidylcholine by ABCG1."
No.
Sentence
Comment
250
It was reported that functional NBD is necessary for the trafficking of ABCG1 to the plasma membrane, because the G121A mutation within the WalkerA motif of ABCG1 abolished the cell surface expression (13).
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ABCG1 p.Gly121Ala 16702602:250:114
status: VERIFIED248 It was reported that functional NBD is necessary for the trafficking of ABCG1 to the plasma membrane, because the G121A mutation within the WalkerA motif of ABCG1 abolished the cell surface expression (13).
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ABCG1 p.Gly121Ala 16702602:248:114
status: NEW