ABCMdb

PMID: 15994327

Vaughan AM, Oram JF
ABCG1 redistributes cell cholesterol to domains removable by high density lipoprotein but not by lipid-depleted apolipoproteins.
J Biol Chem. 2005 Aug 26;280(34):30150-7. Epub 2005 Jun 30., 2005-08-26 [PubMed]

Sentences

No. Mutations Sentence Comment

47 ABCG1 p.Gly121Ala Site-directed mutagenesis of an amino acid residue common to all ABC transporter nucleotide binding domains (NCBI conserved domains data base, cd00267.1) (28) within the Walker A motif (29) of ABCG1 (glycine121 to alanine, GenBankTM accession number NP_004906) was carried out using the QuikChange XL site-directed mutagenesis kit (Stratagene, CA). Login to comment 140 ABCG1 p.Gly121Ala The G121A mutation almost completely abolished the ability of ABCG1 to redistribute cholesterol to oxidase- and ACAT-accessible pools and to promote cholesterol efflux to HDL (Fig. 5D). Login to comment 160 ABCG1 p.Gly121Ala There was no significant decrease in cholesterol content during the HDL incubation in the mock-transfected cells or in the ABCG1 G121A transfectants (Fig. 6C). Login to comment 181 ABCG1 p.Gly121Ala C, equal amounts of cell protein from BHK cells transfected with FLAG-tagged wild type ABCG1 (WT) or a NBD ABCG1 mutant (G121A) were resolved by SDS-PAGE in the presence or absence of beta-mercaptoethanol (BME), and ABCG1 was detected by immunoblot analysis using an anti-FLAG antibody. Login to comment 185 ABCG1 p.Gly121Ala *, p Ͻ 0.03 versus mock- or ABCG1-G121A-transfected cells. Login to comment 186 ABCG1 p.Gly121Ala E, cell-surface (biotinylated) FLAG-tagged ABCG1 and ABCG1 G121A were detected by a streptavidin blotting assay following immunoprecipitation with an anti-FLAG antibody. Login to comment