ABCMdb

PMID: 9891078

Zhou Y, Gottesman MM, Pastan I
Studies of human MDR1-MDR2 chimeras demonstrate the functional exchangeability of a major transmembrane segment of the multidrug transporter and phosphatidylcholine flippase.
Mol Cell Biol. 1999 Feb;19(2):1450-9., [PubMed]

Sentences

No. Mutations Sentence Comment

49 ABCB1 p.Gln330Asn ABCB1 p.Leu332Met ABCB1 p.Val331Ala MDR1NAM was created by introducing mutations Q330N, V331A, and L332M into MDR1 by PCR mutagenesis. Login to comment 169 ABCB1 p.Gln330Asn ABCB1 p.Leu332Met ABCB1 p.Val331Ala To confirm the significance of Q330, V331, and L332 in TM6, we generated a MDR1 mutant containing the mutations Q330N, V331A, and L332M. Login to comment 226 ABCB1 p.Gln330Asn ABCB1 p.Leu332Met ABCB1 p.Val331Ala One is that the substitutions Q330N, V331A, and L332M in MDR1 significantly decreased overall MDR1 multidrug transporter activity. Login to comment 241 ABCB1 p.Gln330Asn ABCB1 p.Leu332Met ABCB1 p.Val331Ala Thus, the triple mutation Q330N, V331A, and L332M appeared to have a synergistic effect. Login to comment 262 ABCB1 p.Gln330Asn ABCB1 p.Leu332Met ABCB1 p.Val331Ala Since the UIC2 epitope depends on a particular P-gp conformation (24), the decreased level of UIC2 recognition may indicate a conformational change in MDR1 caused by mutations Q330N, V331A, and L332M. Login to comment