ABCMdb

PMID: 9755153

Schwanstecher M, Sieverding C, Dorschner H, Gross I, Aguilar-Bryan L, Schwanstecher C, Bryan J
Potassium channel openers require ATP to bind to and act through sulfonylurea receptors.
EMBO J. 1998 Oct 1;17(19):5529-35., [PubMed]

Sentences

No. Mutations Sentence Comment

112 ABCC8 p.Lys719Ala ABCC8 p.Lys1384Met For example, the substitution of an alanine for the conserved lysine in the Walker A motif in NBF1 (K719A) abolished diazoxide-induced activation of SUR1/ KIR6.2 channels, whereas substitution of a methionine at the equivalent position in NBF2 (K1384M) had a more subtle effect, eliminating activation by diazoxide in the presence of low (10 µM), but not high (100 µM) [ATP] (Gribble et al., 1997). Login to comment 114 ABCC8 p.Lys719Arg ABCC8 p.Lys1384Arg However, by analogy with the results seen with SUR2B, substitution of a lysine for an arginine in either NBF of SUR1 (K719R and/or K1384R) eliminates diazoxide binding and induces a complete loss of activation of SUR1/KIR6.2 channels (results not shown). Login to comment 115 ABCC8 p.Lys1384Met On the other hand, substitution of a methionine in NBF2 of SUR1 (K1384M) does not reduce KCO binding at saturating ATP, but decreases the affinity for ATP (M.Schwanstecher et al., unpublished data). Login to comment