PMID: 9711563

Greenberger LM
Identification of drug interaction sites in P-glycoprotein.
Methods Enzymol. 1998;292:307-17., [PubMed]
Sentences
No. Mutations Sentence Comment
199 ABCB1 p.Phe335Ala
X
ABCB1 p.Phe335Ala 9711563:199:74
status: NEW
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 Furthermore, alterations in photoaffinity labeling were observed with the F335A mutants.3° This does not exclude the fact that other regions in P-glycoprotein play a functional role in drug binding or transport. Login to comment 201 ABCB1 p.Gly185Val
X
ABCB1 p.Gly185Val 9711563:201:34
status: NEW
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 In particular, point mutations in G185V in human P-glycoprotein (immediately before TM3)31 and $941F or $939F in TMll of mouse P-glycoprotein encoded by rndrl and mdr3, respectively, altered drug resistance profiles32and the ability to photolabel the mutant proteins.33'34In summary, these data suggest that different drugs have overlapping but distinct binding sites, and some of the drug-binding sites may exist outside the photolabeling regions. Login to comment