ABCMdb

PMID: 9675026

Hodgson RJ, Plaxton WC
Purification and characterization of cytosolic fructose-1, 6-bisphosphate aldolase from endosperm of germinated castor oil seeds.
Arch Biochem Biophys. 1998 Jul 15;355(2):189-96., [PubMed]

Sentences

No. Mutations Sentence Comment

165 ABCD1 p.Arg17Trp Immunoremoval was performed with 0.008 unit of each ALD as described under Materials and Methods. TABLE II Amino Acid Composition of Germinated Castor Seed Cytosolic Aldolase Amino acid Residues per 40-kDa subunit Asx 35 Thr 23 Ser 14 Glx 46 Pro 20 Gly 34 Ala 46 Cys 7 Val 24 Met 2 Ile 14 Leu 39 Tyr 12 Phe 6 His 5 Lys 22 Arg 17 Trp 1 (4, 6, 8, 9, 11) the purified COS ALDc showed a very broad pH/activity profile for the forward reaction, with a maximum occurring at about pH 7.2 and near-maximal activity occurring in the pH range 6.4 to 8.0. Login to comment 166 ABCD1 p.Arg17Trp Immunoremoval was performed with 0.008 unit of each ALD as described under Materials and Methods. TABLE II Amino Acid Composition of Germinated Castor Seed Cytosolic Aldolase Amino acid Residues per 40-kDa subunit Asx 35 Thr 23 Ser 14 Glx 46 Pro 20 Gly 34 Ala 46 Cys 7 Val 24 Met 2 Ile 14 Leu 39 Tyr 12 Phe 6 His 5 Lys 22 Arg 17 Trp 1 (4, 6, 8, 9, 11) the purified COS ALDc showed a very broad pH/activity profile for the forward reaction, with a maximum occurring at about pH 7.2 and near-maximal activity occurring in the pH range 6.4 to 8.0. Login to comment