ABCMdb

PMID: 9671706

Hall RA, Ostedgaard LS, Premont RT, Blitzer JT, Rahman N, Welsh MJ, Lefkowitz RJ
A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins.
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8496-501., 1998-07-21 [PubMed]

Sentences

No. Mutations Sentence Comment

43 ABCC7 p.Lys1468* The following proteins were in vitro translated by using the TNT system (Promega) in the presence of [35 S]methionine: full-length CFTR-4, CFTR-K1468X, or fusion proteins of CFTR cloned into the pET33b vector (Novagen) (C-terminal amino acids 1387-1480 or N-terminal amino acids 1-80). Login to comment 111 ABCC7 p.Lys1468* Conversely, neither the N-terminal portion of CFTR nor a C-terminally truncated mutant CFTR (K1468X) exhibited detectable binding to NHERF. Login to comment 149 ABCC7 p.Ser1455* Interestingly, a naturally occurring truncation of the tail of CFTR (S1455X) has been recently reported (24). Login to comment 163 ABCC7 p.Lys1468* Full-length CFTR and CFTR C terminus bound avidly to the NHERF but CFTR N terminus and CFTR K1468X did not detectably bind. Login to comment