ABCMdb

PMID: 9643548

Poncelet M, Cassier-Chauvat C, Leschelle X, Bottin H, Chauvat F
Targeted deletion and mutational analysis of the essential (2Fe-2S) plant-like ferredoxin in Synechocystis PCC6803 by plasmid shuffling.
Mol Microbiol. 1998 May;28(4):813-21., [PubMed]

Sentences

No. Mutations Sentence Comment

100 ABCC8 p.Glu88Ala Three of the fedI mutant alleles presently tested encoded a protein with a single amino acid substitution (C85V, E88A or E93A), whereas the last one, originating from a PCR artifact, directed the synthesis of a FedI product with a C-terminal extension (KGNLSSLA). Login to comment 107 ABCC8 p.Glu88Ala Similarly, the E88A mutant ferredoxin (pMP3 plasmid) behaved essentially as the wild-type protein, ruling out the contribution of E-88 to an important electrostatic interaction with PSI. Login to comment 133 ABCC8 p.Glu88Ala We have found that the independent E88A and E93A mutations of fedI, each removing one negative charge, had little effect on the tested properties of ferredoxin (electrophoretic mobility, reduction by PSI). Login to comment 158 ABCC8 p.Glu88Ala Similarly, the mutant alleles of the Synechocystis fedI gene carrying the amino acid substitutions C85V, E88A and E93A were synthesized by PCR, using the NdeI creating primer and any of the following XhoI mutagenic primers 5Ј-CCCA- TGGCCACTCGAGTTACCCTTAGTAGAGGTCTTCTTC-3Ј, 5Ј-CCCATGGCCACTCGAGTTACCCTTAGTAGAGGTCTG- CTTCTTTGTG-3Ј or 5Ј-CCATGGCCACTCGAGTTACCCT- TAGTAGAGGTCTTCTTCTTGTGGGTTGCAATGG-3Ј, as required. Login to comment