ABCMdb

PMID: 9382893

Shyng S, Ferrigni T, Nichols CG
Regulation of KATP channel activity by diazoxide and MgADP. Distinct functions of the two nucleotide binding folds of the sulfonylurea receptor.
J Gen Physiol. 1997 Dec;110(6):643-54., [PubMed]

Sentences

No. Mutations Sentence Comment

139 ABCC8 p.Lys719Ala ABCC8 p.Lys1384Met Gribble et al. (1997) have recently shown that mutation of the conserved lysine residues in the Walker A motifs of either NBF1 (mutation K719A) or NBF2 (mutation K1384M), which are predicted to reduce ATP hydrolytic activity (Azzaria et al., 1989; Carson et al., 1995; Ko and Pedersen, 1995), can block the stimulatory effect of MgADP. Login to comment 140 ABCC8 p.Lys719Ala ABCC8 p.Lys1384Met In that study, diazoxide stimulated K1384M (NBF2 mutant) channels, but failed to stimulate K719A (NBF1 mutant) channels, leading to the suggestion that hydrolysis at NBF1 was most critical for diazoxide stimulation. Login to comment