ABCMdb

PMID: 9211861

Falson P, Menguy T, Corre F, Bouneau L, de Gracia AG, Soulie S, Centeno F, Moller JV, Champeil P, le Maire M
The cytoplasmic loop between putative transmembrane segments 6 and 7 in sarcoplasmic reticulum Ca2+-ATPase binds Ca2+ and is functionally important.
J Biol Chem. 1997 Jul 11;272(28):17258-62., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCC8 p.Asp818Ala ABCC8 p.Asp818Ala Two cluster mutants of Ca2؉ -ATPase, D813A/D818A and D813A/D815A/D818A, expressed in the yeast Saccharomyces cerevisiae, were found to have a very low Ca2؉ -ATPase activity. Login to comment 46 ABCC8 p.Asp818Ala ABCC8 p.Asp818Ala One single mutation E309Q, one double mutation D813A/D818A (referred to later as ADA), and one triple mutation D813A/D815A/D818A (AAA) were introduced. Login to comment 121 ABCC8 p.Asp818Ala ABCC8 p.Asp818Ala Preliminary attempts with molecular modeling of the Ca2ϩ -ATPase 808-818 sequence by energy minimization according to the ␣-lactalbumin Ca2ϩ binding site suggested that the side chains of Asp-813 and Asp-818, together with the peptide carbonyl group between Asp-815 and Ile-816 could be involved in Ca2ϩ binding to the L6-7 loop.2 We then took advantage of the functional expression of Ca2ϩ -ATPase in yeast (53) to test the activity of two cluster mutants in this region, i.e. D813A/D818A (ADA) and D813A/D815A/D818A (AAA). Login to comment 142 ABCC8 p.Asp818Ala ABCC8 p.Asp818Ala The reaction medium contained 50 ␮g/ml yeast membranes with wild type or mutant Ca2ϩ -ATPases: D813A/D818A (ADA), D813A/D815A/D818A (AAA), or E309Q. Login to comment