PMID: 8918588

Moss RB, Bocian RC, Hsu YP, Dong YJ, Kemna M, Wei T, Gardner P
Reduced IL-10 secretion by CD4+ T lymphocytes expressing mutant cystic fibrosis transmembrane conductance regulator (CFTR).
Clin Exp Immunol. 1996 Nov;106(2):374-88., [PubMed]
Sentences
No. Mutations Sentence Comment
159 ABCC7 p.Arg117His
X
ABCC7 p.Arg117His 8918588:159:271
status: NEW
view ABCC7 p.Arg117His details
 Southern hybridization blots of reverse transcriptase-polymerase chain reaction (RT-PCR) products of CFTR derived from cDNAs prepared from the T84 adenocarcinoma cell line (lane 1), water control (lane 2), a F508 homozygous CF-derived CD4‡ T cell clone (lane 3), a R117H homozygous CF-derived CD4‡ T cell clone (lane 4), two normal (wild type CFTR) T cell clones (lanes 5 and 6), a neuroblastoma cell line (lane 7), the HeLa cell line (lane 8), another water control (lane 9), and CFTR plasmid (lane 10). Login to comment 281 ABCC7 p.Arg117His
X
ABCC7 p.Arg117His 8918588:281:758
status: NEW
view ABCC7 p.Arg117His details
 IL-10 production by concanavalin A-activated T cell clones Group IL-10 P Cystic fibrosis, total (n ˆ 83)* 684 6116 0.05 Control, total (n ˆ 97) 1565 6401 Atopic (n ˆ 92) 672 6173 0.026 Non-atopic (n ˆ 88) 1667 6417 CF, atopic (n ˆ 34) 417 6120 0.056 CF, non-atopic (n ˆ 49) 869 6175 Control, atopic (n ˆ 58) 821 6264 0.023 Control, non-atopic (n ˆ 39) 2670 6895 Non-atopic, control (n ˆ 39) 2670 6895 0.031 Non-atopic, CF (n ˆ 49) 869 6175 Atopic, control (n ˆ 58) 821 6264 NS Atopic, CF (n ˆ 34) 417 6120 Values are pg/ml per 106 cells, mean 6s:e:m: * Includes two clones from a non-atopic individual with congenital bilateral absence of the vas deferens but not clinical CF who is homozygous for R117H CFTR alleles. Login to comment