ABCMdb

PMID: 8790436

Ruetz S, Delling U, Brault M, Schurr E, Gros P
The pfmdr1 gene of Plasmodium falciparum confers cellular resistance to antimalarial drugs in yeast cells.
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9942-7., [PubMed]

Sentences

No. Mutations Sentence Comment

38 ABCC1 p.Ser1034Cys ABCC1 p.Ser1034Cys Mutant variants ofpftndrl bearing a single Ser -> Cys substitution at position 1034 (S1034C, clone M1034), a single Asn -> Asp substitution at position 1042 (N1042D, clone M1042), or both mutations (S1034C/N1042D; clone DM) were generated by site-directed mutagenesis (26). Login to comment 88 ABCC1 p.Ser1034Cys ABCB4 p.Asn1042Asp The expression of the wild-type P. falciparum Pgh-1 protein (A) and mutant variants bearing single S1034C (B) or N1042D (C) mutations or both (D) in permeabilized yeast S. cerevisiae spheroplasts is shown by immunofluorescence using a specific antiserum (rabbit anti-Pgh-1) at a 1:100 dilution, followed by an fluorescein isothiocyanate-coupled goat anti-rabbit antibody at a dilution of 1:800. Login to comment 94 ABCC1 p.Ser1034Cys ABCC1 p.Ser1034Cys ABCB4 p.Asn1042Asp ABCB4 p.Asn1042Asp Time-dependent cell growth in absence (solid symbols) or presence (open symbols) of MFQ at 150 ,ug/ml was established for JPY201 transformants expressing wild-type Pgh-1, wild-type Mdr3 (Mdr3S), or Pgh-1 variants bearing single S1034C (M1034) or single N1042D (M1042) mutations or both mutations (S1034C/N1042D; DM) in predicted TM11 or a mutant variant of Mdr3 (S939F; Mdr3F). Login to comment 97 ABCC1 p.Ser1034Cys ABCC1 p.Ser1034Cys ABCB4 p.Asn1042Asp ABCB4 p.Asn1042Asp JPY201 cells expressing wild-type Pgh-1, wild-type Mdr3 (Mdr3S), or mutant Pgh-1 variants bearing single S1034C (M1034), single N1042D M1042) mutations, or both mutations (S1034C/N1042D; DM) were evaluated for growth in increasing concentrations of MFQ. Login to comment 133 ABCC1 p.Ser1034Cys ABCB4 p.Asn1042Asp JPY201 cells expressing either wild-type Pgh-1 or a double mutant Pgh-1 variant (S1034C/ N1042D; DM) were incubated with QA. Login to comment