ABCMdb

PMID: 8621506

Shani N, Sapag A, Valle D
Characterization and analysis of conserved motifs in a peroxisomal ATP-binding cassette transporter.
J Biol Chem. 1996 Apr 12;271(15):8725-30., [PubMed]

Sentences

No. Mutations Sentence Comment

98 ABCB1 p.Leu70Gly We also changed a nonconserved amino acid near the loop1 motif (L70) to glycine because in previous work we suggested that this residue might play a role in fatty acid binding (11) Analysis of the loop1 Protein Motif-Alterations of the conserved loop1 arginine have an effect on Pxa1p function (Fig. 6); the R108L mutation has a null phenotype, as measured by growth on oleic acid and the beta-oxidation assay; R108K has an intermediate phenotype in both assays (approximately 50% of wild type), whereas the growth phenotype of L70G was indistinguishable from wild type (Fig. 6) (beta-oxidation not performed). Login to comment 112 ABCB1 p.Leu70Gly A, phenotypes of pxa1::LEU2 knockout (ko) yeast expressing (from left to right): wild type (wt), L70G, R108L, and R108K alleles of PXA1 or vector alone. 10-fold dilutions (top to bottom) of the different cells were inoculated on medium containing 0.1% oleic acid as a sole carbon source. Login to comment