PMID: 8516295

Lalo D, Carles C, Sentenac A, Thuriaux P
Interactions between three common subunits of yeast RNA polymerases I and III.
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5524-8., [PubMed]
Sentences
No. Mutations Sentence Comment
62 ABCC8 p.Val86Arg
X
ABCC8 p.Val86Arg 8516295:62:201
status: NEW
view ABCC8 p.Val86Arg details
 The remaining three mutations are ts: rpcl9-Y78R brought the AC19 sequence closer to the consensus a motif by restoring a highly conserved pair of arginines (see Fig. 1A), whereas rpcl9-G73D and rpcl9-V86R corresponded to the ts mutations rpc40-A64D and rpc40-V78R. Login to comment 113 ABCC8 p.Val86Arg
X
ABCC8 p.Val86Arg 8516295:113:493
status: NEW
view ABCC8 p.Val86Arg details
 Growth phenotype of rpc40 and rpcl9 mutants Triplet Growth at Mutant substitution 20°C 300C 37°C Phenotype* RPC40 DLY10 (A64D) GCG/GAC + ++ - ts DLY62 (N65D) AAT/GAT + + + 1 DLY59 (N65H) AAT/CAT + ++ ++ 1 DLY28 (N65Q) AAT/CAA - ++ +++ Cs DLY18 (R68K) CGT/AAG + ++ ++ 1 DLY48 (R69K) CGT/AAG + + + 1 DLY78 (R69C) CGT/TGT - + - cs,ts DLY7 (V78R) GTG/AGG ++ +++ - ts DLY7Ct (V78R) GTG/AGG + + + + + - ts RPC19 DLY202 (G73D) GGA/GAT ++ +++ - ts DLY208 (Y78R) TAC/CGC ++ +++ ++ ts DLY210 (V86R) GTA/CGA + + + + + + ts Except for strain DLY7C, the mutations were borne on the centromeric plasmid p7040 (URA3 RPC40) or p3519 (TRPI RPCI9) in a chromosomal rpc4O-A::HIS3 or rpc19-Ai::HIS3 context. Login to comment