ABCMdb

PMID: 26259795

Souza AC, Fuchs BB, Pinhati HM, Siqueira RA, Hagen F, Meis JF, Mylonakis E, Colombo AL
Candida parapsilosis Resistance to Fluconazole: Molecular Mechanisms and In Vivo Impact in Infected Galleria mellonella Larvae.
Antimicrob Agents Chemother. 2015 Oct;59(10):6581-7. doi: 10.1128/AAC.01177-15. Epub 2015 Aug 10., [PubMed]

Sentences

No. Mutations Sentence Comment

108 ABCB1 p.Thr591Cys GQ302972) revealed the presence of a silent mutation (T591C) and a missense mutation (A395T) that led to a Y132F amino acid substitution and a changeintheproteinsequence(Table2).Singleallelemutations(i.e., heterozygous for the mutation) were not observed; there were only point mutations in both alleles (i.e., homozygous for the mutation). Login to comment 110 ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys ABCB1 p.Thr591Cys To investigate if changes in the expression patterns of the ERG11 gene, the ABC transporter TABLE 2 In vitro activities of four antifungal agents against nine clinical isolates of C. parapsilosis (sensu stricto) in the CLSI broth microdilution assay complemented by ERG11 sequence analysis and expression of the ERG11, CDR1, and MDR1 genes C. parapsilosis (sensu stricto) isolate MIC (mg/liter) Mutations in ERG11 gene Avg cDNA level (SD)a FLC VRC ANF AMB ERG11 CDR1 MDR1 LEMI 8646 8 1 2 0.25 T591C, A395T 7.4 (0.3) 9.2 (0.6) 1.5 (0.08) LEMI 8650 8 0.5 2 0.25 T591C, A395T 3.7 (0.1) 4.8 (0.1) 0.9 (0.06) LEMI 8653 16 0.5 2 0.25 T591C, A395T 1.5 (0.1) 4.0 (0.3) 0.9 (0.1) LEMI 8655 16 0.5 1 0.25 T591C, A395T 3.3 (0.2) 3.4 (0.1) 0.8 (0.2) LEMI 8657 16 0.5 2 0.125 T591C, A395T 3.9 (0.3) 3.7 (0.2) 1.0 (0.06) LEMI 8662 16 0.5 2 0.25 T591C, A395T 5.7 (0.5) 5.7 (0.5) 1.3 (0.2) LEMI 8379 8 0.5 2 0.25 T591C, A395T 4.0 (0.3) 3.3 (0.3) 0.8 (0.04) LEMI 8382 8 0.5 2 0.25 T591C, A395T 7.3 (0.4) 7.3 (0.5) 1.2 (0.06) LEMI 8383 8 0.5 1 0.25 T591C, A395T 4.4 (0.4) 7.6 (0.6) 1.0 (0.1) ATCC 22019 0.5 0.5 1 0.125 NDb 1.0 (0.00) 1.0 (0.07) 1.0 (0.07) a cDNA levels were calculated relative to average levels of cDNA obtained for the wild-type strain. Login to comment