PMID: 25600711

Pan L, Aller SG
Equilibrated atomic models of outward-facing P-glycoprotein and effect of ATP binding on structural dynamics.
Sci Rep. 2015 Jan 20;5:7880. doi: 10.1038/srep07880., [PubMed]
Sentences
No. Mutations Sentence Comment
196 ABCB1 p.Leu975Cys
X
ABCB1 p.Leu975Cys 25600711:196:136
status: NEW
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ABCB1 p.Leu332Cys
X
ABCB1 p.Leu332Cys 25600711:196:130
status: NEW
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ABCB1 p.Leu339Cys
X
ABCB1 p.Leu339Cys 25600711:196:96
status: NEW
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ABCB1 p.Phe343Cys
X
ABCB1 p.Phe343Cys 25600711:196:190
status: NEW
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ABCB1 p.Val982Cys
X
ABCB1 p.Val982Cys 25600711:196:102
status: NEW
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ABCB1 p.Val982Cys
X
ABCB1 p.Val982Cys 25600711:196:196
status: NEW
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 Specifically, ATP binding inhibited the crosslink of pairs of human Pgp between TM6 and TM12 at L339C-V982C (mouse L334-V978) and L332C-L975C (mouse L328-L971) but promoted the crosslink of F343C-V982C (mouse F339-V978). Login to comment 203 ABCB1 p.Ile1050Cys
X
ABCB1 p.Ile1050Cys 25600711:203:150
status: NEW
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ABCB1 p.Pro517Cys
X
ABCB1 p.Pro517Cys 25600711:203:129
status: NEW
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 We did not simulate cross-linking per se, but our structure showed direct side-chain contact between the crosslinked pair LSGGQ (P517C) and Walker A (I1050C) (mouse P513-I1046) showing agreement between our homology model and the biochemical data. Login to comment